Transplanted fibroblast cell sheets promote migration of hepatic progenitor cells in the incised host liver in allogeneic rat model
Transplanted fibroblast cell sheets promote migration of hepatic progenitor cells in the incised host liver in allogeneic rat model
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DOI：
10.1002/term.1718
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发表时间：
2012-02
影响因子：
3.3
通讯作者：
I. Muraoka;M. Takatsuki;Y. Sakai;T. Tomonaga;A. Soyama;M. Hidaka;Y. Hishikawa;T. Koji;R. Utoh;K. Ohashi;T. Okano;T. Kanematsu;S. Eguchi
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文献类型：
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作者：
I. Muraoka;M. Takatsuki;Y. Sakai;T. Tomonaga;A. Soyama;M. Hidaka;Y. Hishikawa;T. Koji;R. Utoh;K. Ohashi;T. Okano;T. Kanematsu;S. Eguchi
细胞片工程已被认为是组织工程领域的一种新的和有价值的方法。本研究的目的是探索一种在肝脏中诱导肝祖细胞和胆管结构的方法。将Sprague-Dawley大鼠真皮成纤维细胞（DF）片移植到F344裸大鼠肝脏的切开表面。在对照组中，制作切口而不移植DF片。在DF片移植部位观察到胆管（BD）样结构和未成熟肝细胞样细胞。这些BD样结构为细胞角蛋白8阳性，而肝细胞样细胞也为OV 6阳性和甲胎蛋白阳性。肝切除对肝前体细胞的增殖和分化无明显影响。我们还通过电穿孔将用编码增强型黄色荧光蛋白靶标的质粒转染的DF片移植到线粒体（pEYFP-Mito），并发现新结构是pEYFP-Mito阴性的。在将DF片移植到切开的肝脏表面后，我们观察到新的BD样结构和未成熟肝细胞，并阐明这些BD样结构和肝细胞样细胞的来源是受体肝脏。本研究描述了在肝损伤部位诱导的肝分化过程的一个方面。版权所有© 2013约翰威利父子有限公司.
Cell sheet engineering has been noted as a new and valuable approach in the tissue‐engineering field. The objective of this study was to explore a procedure to induce hepatic progenitor cells and biliary duct structures in the liver. Sprague–Dawley rat dermal fibroblast (DF) sheets were transplanted into the incised surface of the liver of F344 nude rats. In the control group, an incision was made without transplantation of the DF sheets. Bile duct (BD)‐like structures and immature hepatocyte‐like cells were observed in the DF sheet transplant sites. These BD‐like structures were cytokeratin‐8‐positive, while the hepatocyte‐like cells were both OV‐6‐positive and α‐fetoprotein‐positive as well. The proliferation and differentiation of liver progenitor cells were not influenced by hepatectomy. We also transplanted DF sheets transfected with a plasmid encoding the enhanced yellow fluorescent protein target to mitochondria (pEYFP–Mito) by electroporation, and found that the new structures were pEYFP–Mito‐negative. We observed new BD‐like structures and immature hepatocytes after transplantation of DF sheets onto incised liver surfaces, and clarified that the origin of these BD‐like structures and hepatocyte‐like cells was the recipient liver. The present study described an aspect of the hepatic differentiation process induced at the site of liver injury. Copyright © 2013 John Wiley & Sons, Ltd.