Identification of sam4 as a rad24 Allele in Schizosaccharomyces pombe
Identification of sam4 as a rad24 Allele in Schizosaccharomyces pombe
复制标题
粟酒裂殖酵母中 sam4 鉴定为 rad24 等位基因
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
M. Kawamukai
中科院分区：
文献类型：
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作者：
Yasuo Oowatari;Kenichi Toma;F. Ozoe;M. Kawamukai
裂变酵母需要营养饥饿来将有丝分裂细胞周期转换为性别分化，但我们已经分离出9个等位基因的sam突变体在没有饥饿条件的情况下交配。这些突变体有助于理解细胞感知营养饥饿和改变细胞周期的机制。为了鉴定相同等位基因，我们首先寻找原始相同表型以外的表型。研究发现，9个突变体均对1 M KCl敏感，sam2、sam3、sam4和sam9对0.1 M CaCl2敏感，只有sam4突变体对150 J/m2 UV敏感。sam4的这种特殊表型提示sam4可能是编码14-3-3蛋白的rad24的等位基因。事实上，Rad24蛋白在sam4中消失，Rad24 mRNA在sam4中没有转录。此外，在sam4的rad24位点发现了Gln变为停止密码子的突变。因此，我们得出结论，sam4是rad24的等位基因。我们还发现过表达rad24或rad25 （rad24的平行基因）对sam1有抑制作用，sam1既不是rad24的等位基因，也不是rad25的等位基因。因此14-3-3蛋白深入参与了有丝分裂细胞周期向裂变酵母性分化的转换。
Fission yeast requires nutritional starvation to switch the mitotic cell cycle to sexual differentiation, but sam mutants, of which we had isolated nine alleles, mate without the starvation condition. These mutants are useful for understanding the mechanism underlying the way cells sense nutritional starvation and change the cell cycle. To identify the sam allele, we first sought phenotypes other than the original sam phenotype. We found that all nine sam mutants were sensitive to 1 M KCl, that sam2, sam3, sam4 and sam9 were sensitive to 0.1 M CaCl2, and that only the sam4 mutant was sensitive to 150 J/m2 UV. This peculiar phenotype of sam4 suggested to us that sam4 might be an allele of rad24, which encodes a 14-3-3 protein. In fact, the Rad24 protein disappeared in sam4 and the rad24 mRNA was not transcribed in sam4. In addition, the mutation that changed Gln to a stop codon was found in the rad24 locus of sam4. Hence we concluded that sam4 is an allele of rad24. We also found that over-expression of rad24 or rad25 (a paralog of rad24) has a suppressive effect on sam1, and that sam1 was not an allele of rad24 nor rad25. Thus 14-3-3 proteins are deeply involved in the switching of the mitotic cell cycle to the sexual differentiation of fission yeast.
影响因子：
3.9
作者：
Hoffman, CS
通讯作者：
Hoffman, CS