FUNCTIONAL-ANALYSIS OF THE TRANSCRIPTIONAL PROMOTER FOR THE CYP1A1 GENE
FUNCTIONAL-ANALYSIS OF THE TRANSCRIPTIONAL PROMOTER FOR THE CYP1A1 GENE
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DOI：
10.1128/mcb.10.10.5098
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发表时间：
1990-10-01
影响因子：
5.3
通讯作者：
WHITLOCK, JP
中科院分区：
文献类型：
--
作者：
JONES, KW;WHITLOCK, JP
In mouse hepatoma cells, the environmental contaminant 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) increases the transcription rate of the CYP1A1 gene, which encodes a cytochrome P-450 enzyme. In this study, we analyzed the DNA region immediately upstream of the CYP1A1 gene. A domain that extends upstream to nucleotide -166 was found to function as a transcriptional promoter. The promoter was silent when uncoupled from the dioxin-responsive enhancer located farther upstream. DNase footprinting experiments indicated that nuclear proteins interact with distinct domains of the promoter in a TCDD-independent fashion. Mutational analyses indicated that the CYP1A1 promoter contains at least three functional domains, including a TATAAA sequence, a CCAAT box transcription factor/nuclear factor I-like recognition motif, and a guanine-rich G box.
