Ginkgo biloba extract (EGb 761) induces apoptosis by the activation of caspase-3 in oral cavity cancer cells
Ginkgo biloba extract (EGb 761) induces apoptosis by the activation of caspase-3 in oral cavity cancer cells
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DOI：
10.1016/j.oraloncology.2004.09.013
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发表时间：
2005-04-01
期刊：
影响因子：
4.8
通讯作者：
Yoo, JB
中科院分区：
文献类型：
--
作者：
Kim, KS;Rhee, KH;Yoo, JB
我们研究了银杏叶提取物（EGb 761）是否诱导口腔癌细胞凋亡，并试图表征EGb 761激活的细胞凋亡途径。250 μ g/ml的EGb 761可抑制SCC 1483口腔癌细胞的增殖。250 μ g/ml EGb 761孵育24小时后观察到细胞凋亡，并呈时间和剂量依赖性。细胞凋亡通过DNA片段化和PARP切割来证实。与半胱天冬酶抑制剂（z-VAD-favorite）共同处理可抑制EGb 761诱导的细胞凋亡和PARP裂解。Caspase-3活性被EGb 761上调，但通过与z-VAD-fetamine共处理降低至对照水平。综上所述，EGb 761诱导口腔癌细胞凋亡，caspase-3在该凋亡中被激活。因此，EGb 761可能是一种潜在的口腔癌化学预防剂。(c)2004爱思唯尔有限公司保留所有权利。
We have investigated whether Ginkgo biloba extract (EGb 761) induces apoptosis of oral cavity cancer cells and attempted to characterize the apoptotic pathway activated by EGb 761. The inhibition of SCC 1483 oral cavity cancer cells proliferation was noted from 250 mu g/ml of EGb 761. Apoptosis was observed after 24h of incubation with 250 mu g/ml EGb 761 and occurred in a time- and dose-dependent manner. Apoptosis was confirmed by DNA fragmentation and PARP cleavage. Co-treatment with the caspase inhibitor (z-VAD-fmk) inhibited apoptosis and PARP cleavage induced by EGb 761. Caspase-3 activity was upregulated by EGb 761 but reduced to the control level by co-treating with z-VAD-fmk. In summary, EGb 761 induces apoptosis of oral cavity cancer cells and caspase-3 is activated in this apoptosis. Therefore, EGb 761 may be considered as a possible chemopreventive agent against oral cavity cancer. (c) 2004 Elsevier Ltd. All rights reserved.