Phage Therapy as a Promising New Treatment for Lung Infection Caused by Carbapenem-Resistant Acinetobacter baumannii in Mice
Phage Therapy as a Promising New Treatment for Lung Infection Caused by Carbapenem-Resistant Acinetobacter baumannii in Mice
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噬菌体疗法作为治疗小鼠碳青霉烯类耐药鲍曼不动杆菌引起的肺部感染的一种有前途的新疗法
DOI：
10.3389/fmicb.2017.02659
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发表时间：
2018-01-09
影响因子：
5.2
通讯作者：
He, Ping
中科院分区：
文献类型：
--
作者：
Hua, Yunfen;Luo, Tingting;He, Ping
碳青霉烯类耐药鲍曼不动杆菌（CRAB）是与医疗相关感染相关的主要病原体，其稳定发展已超出抗生素控制范围。裂解性噬菌体具有感染和裂解特定细菌的特性，已被用作传统抗生素的潜在替代品，以解决多重耐药细菌感染。在这里，我们分离了A。鲍曼不动杆菌特异性溶菌素，并评估其对CRAB临床菌株引起的肺部感染的潜在治疗作用。4种裂解酶谱对CRAB临床分离株的联合裂解率为87.5%（42/48）。基因组测序和分析表明，噬菌体SH-Ab 15519是一种新的噬菌体，不含毒力或抗生素抗性基因。体内研究表明，鼻内给予噬菌体SH-Ab 15519可以有效地拯救小鼠免于致死性A.鲍曼不动杆菌肺部感染而无有害副作用。我们的工作探讨了潜在的使用，作为一种替代治疗剂对肺部感染引起的CRAB菌株。
Carbapenem-resistant Acinetobacter baumannii (CRAB) which is noted as a major pathogen associated with healthcare-associated infections has steadily developed beyond antibiotic control. Lytic bacteriophages with the characteristics of infecting and lysing specific bacteria have been used as a potential alternative to traditional antibiotics to solve multidrug-resistant bacterial infections. Here, we isolated A. baumannii-specific lytic phages and evaluated their potential therapeutic effect against lung infection caused by CRAB clinical strains. The combined lysis spectrum of four lytic phages’ ranges was 87.5% (42 of 48) against CRAB clinical isolates. Genome sequence and analysis indicated that phage SH-Ab15519 is a novel phage which does not contain the virulence or antibiotic resistance genes. In vivo study indicated that phage SH-Ab15519 administered intranasally can effectively rescue mice from lethal A. baumannii lung infection without deleterious side effects. Our work explores the potential use of phages as an alternative therapeutic agent against the lung infection caused by CRAB strains.