An amperometric chloramphenicol immunosensor based on cadmium sulfide nanoparticles modified-dendrimer bonded conducting polymer
An amperometric chloramphenicol immunosensor based on cadmium sulfide nanoparticles modified-dendrimer bonded conducting polymer
复制标题
DOI：
10.1016/j.bios.2009.12.024
复制
发表时间：
2010-03-15
影响因子：
12.6
通讯作者：
Shim, Yoon-Bo
中科院分区：
文献类型：
--
作者：
Kim, Dong-Min;Rahman, Md. Aminur;Shim, Yoon-Bo
将抗氯霉素乙酰转移酶（anti-CAT）抗体共价固定在硫化镉纳米粒子修饰的枝状大分子上，制备了一种电流型氯霉素（CAP）免疫传感器。该枝状大分子与导电聚合物（聚5,2′：5′，2′-噻吩-3′-羧酸（聚ttca））层结合。将AuNPs、树状大分子和CdS纳米颗粒沉积在聚合物层上，以提高传感器探针的灵敏度。采用扫描电子显微镜（SEM）和透射电子显微镜（TEM）测定颗粒大小。利用能谱（EDS）、x射线光电子能谱（XPS）和石英晶体微天平（QCM）技术证实了树突状大分子、CdS和抗cat的固定化。CAP的检测是基于游离和标记的CAP对抗cat活性位点的竞争性免疫相互作用。以联氨作为CAP的标记物，在-0.35 V对Ag/AgCl条件下电化学催化H2O2的还原。在优化的条件下，所提出的免疫传感器在50 pg/mL和950 pg/mL之间的线性检测范围内，检测限为45 pg/mL。免疫传感器在实际肉类样品中进行了检测，用于分析CAP。(C) 2009 Elsevier B.V.。
An amperometric chloramphenicol (CAP) immunosensor was fabricated by covalently immobilizing anti-chloramphenicol acetyl transferase (anti-CAT) antibody on cadmium sulfide nanoparticles (CdS) modified-dendrimer that was bonded to the conducting polymer (poly 5, 2': 5', 2 ''-terthiophene-3'-carboxyl acid (poly-TTCA)) layer. The AuNPs, dendrimers, and CdS nanoparticles were deposited onto the polymer layer in order to enhance the sensitivity of the sensor probes. The particle sizes were determined using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The immobilization of dendrimers, CdS, and anti-CAT were confirmed using energy disruptive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), and quartz crystal microbalance (QCM) techniques. The detection of CAP was based on the competitive immuno-interaction between the free- and labeled-CAP for active sites of the anti-CAT. Hydrazine was used as the label for CAP, and it electrochemically catalyzed the reduction of H2O2 at -0.35 V vs. Ag/AgCl. Under optimized conditions, the proposed immunosensor exhibited a linear range of CAP detection between 50 pg/mL and 950 pg/mL, and the detection limit was 45 pg/mL The immunosensor was examined in real meat samples for the analysis of CAP. (C) 2009 Elsevier B.V. All rights reserved.