A YEAST GENE THAT IS ESSENTIAL FOR RELEASE FROM GLUCOSE REPRESSION ENCODES A PROTEIN-KINASE
A YEAST GENE THAT IS ESSENTIAL FOR RELEASE FROM GLUCOSE REPRESSION ENCODES A PROTEIN-KINASE
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DOI：
10.1126/science.3526554
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发表时间：
1986-09-12
期刊：
影响因子：
56.9
通讯作者：
CARLSON, M
中科院分区：
文献类型：
--
作者：
CELENZA, JL;CARLSON, M
在酿酒酵母中，SNF1基因在碳分解代谢抑制中起核心作用，即SNF1功能是葡萄糖抵抗基因表达所必需的。对克隆的SNF1基因的核苷酸序列进行了测定，预测的氨基酸序列表明SNF1编码一个72,040-Dalton多肽，该多肽与哺乳动物蛋白激酶的保守催化结构域具有显著的同源性。制备了SNF1蛋白的特异性抗血清，用于鉴定SNF1蛋白。在体外的自磷酸化反应中，该蛋白被证明能够将磷酸从三磷酸腺苷转移到丝氨酸和苏氨酸残基。这些发现表明SNF1编码一种蛋白激酶，并提示蛋白磷酸化在真核细胞中碳分解代谢抑制的调节中起关键作用。
TheSNF1gene plays a central role in carbon catabolite repression in the yeastSaccharomyces cerevisiae, namely thatSNF1function is required for expression of glucose-repressible genes. The nucleotide sequence of the clonedSNF1gene was determined, and the predicted amino acid sequence shows thatSNF1encodes a 72,040-dalton polypeptide that has significant homology to the conserved catalytic domain of mammalian protein kinases. Specific antisera were prepared and used to identify theSNF1protein. The protein was shown to transfer phosphate from adenosine triphosphate to serine and threonine residues in an in vitro autophosphorylation reaction. These findings indicate thatSNF1encodes a protein kinase and suggest that protein phosphorylation plays a critical role in regulation by carbon catabolite repression in eukaryotic cells.