Development of a stable Agrobacterium-mediated transformation protocol for Sorghum bicolor Tx430
Development of a stable Agrobacterium-mediated transformation protocol for Sorghum bicolor Tx430
复制标题
DOI：
10.5511/plantbiotechnology.18.0424a
复制
发表时间：
2018-06-01
影响因子：
1.6
通讯作者：
Ezura, Hiroshi
中科院分区：
文献类型：
--
作者：
Sato-Izawa, Kanna;Tokue, Kyoko;Ezura, Hiroshi
高粱是农杆菌介导遗传转化的一种难选作物。对农杆菌介导的高粱遗传转化的几个参数进行了试验，以优化转化频率。本研究利用农杆菌GV2260对高粱Tx430幼胚进行预处理。用热（43摄氏度）处理15或21分钟预处理未成熟胚，并离心导致处理的未成熟胚的转化效率高达1.9%。虽然需要进一步优化以提高转化效率，但本研究有助于高粱植物中感兴趣基因的遗传验证和分子育种。
Sorghum is a recalcitrant crop for Agrobacterium-mediated genetic transformation. Several parameters related to Agrobacterium-mediated transformation were tested to optimize sorghum transformation frequencies. In this study, we evaluated pretreatment of sorghum variety Tx430 immature embryos using Agrobacterium strain GV2260. Pretreatment of immature embryos with heat (43 degrees C) treatment for 15 or 21 min, and centrifugation resulted in a transformation efficiency of up to 1.9% of immature embryos treated. Although further optimization to enhance transformation efficiency is required, this study contributes to the genetic validation of genes of interest and molecular breeding in sorghum plants.