DEGRADATION OF PLANT PATHOGENIC FUNGI BY TRICHODERMA-HARZIANUM
DEGRADATION OF PLANT PATHOGENIC FUNGI BY TRICHODERMA-HARZIANUM
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DOI：
10.1139/m82-110
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发表时间：
1982-01-01
影响因子：
2.8
通讯作者：
HENIS, Y
中科院分区：
文献类型：
--
作者：
ELAD, Y;CHET, I;HENIS, Y
T.哈茨菌分泌的β- 1，3-葡聚糖酶和几丁质酶分别以海带多糖和几丁质为碳源或以白绢病菌细胞壁为碳源时，能向培养基中释放1，3-葡聚糖酶和几丁质酶。哈茨菌在均质S.罗尔夫氏菌核在海带多糖和葡萄糖（3：1，vol/vol）的混合物中生长，葡聚糖酶活性提高了67%。同样，在该真菌的麦麸培养物和接种该培养物的土壤中检测到高溶解活性。拮抗菌攻击菌丝体时，培养基中蛋白酶和脂肪酶活性均呈上升趋势。罗尔夫西。木霉分离物哈茨木霉菌丝体水解酶的产生水平不同。白绢病菌、立枯丝核菌和瓜果腐霉的侵染。这种现象与每种木霉分离物控制相应土传病原体的能力相关。
T. harzianum excreted .beta.-1,3-glucanase and chitinase into the medium when grown on laminarin and chitin, respectively, or on cell walls of the pathogen Sclerotium rolfsii, as sole C source T. harzianum also showed high activity of both enzymes when grown on homogenized S. rolfsii sclerotia. Glucanase activity increased by 67% when the fungus was grown on a mixture of laminarin and glucose (3:1, vol/vol). Similarly, high lytic activity was detected in wheat bran culture of the fungus and in soil inoculated with this culture. Protease and lipase activity were detected in the medium when the antagonist attacked mycelium of S. rolfsii. Isolates of T. harzianum differed in the levels of hydrolytic enzymes produced when mycelium of S. rolfsii, Rhizoctonia solani and Pythium aphanidermatum in soil was attacked. This phenomenon was correlated with the ability of each of the Trichoderma isolates to control the respective soil borne pathogens.