Connexin 43 recruits E-cadherin expression and inhibits the malignant behaviour of lung cancer cells
Connexin 43 recruits E-cadherin expression and inhibits the malignant behaviour of lung cancer cells
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Connexin 43招募E-cadherin表达并抑制肺癌细胞的恶性行为
DOI：
10.2478/v10042-008-0057-9
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发表时间：
2008-01-01
影响因子：
1.5
通讯作者：
Wang, En-Hua
中科院分区：
文献类型：
--
作者：
Xu, Hong-Tao;Li, Qing-Chang;Wang, En-Hua
Cx43和E-cadherin的相互作用可能在肿瘤的发生和恶性行为中起重要作用。在这项研究中，我们研究了连接蛋白43和E-cadherin在人类非小细胞肺癌（NSCLC）中的关系。应用免疫组化方法检测107例NSCLC组织中缝隙连接蛋白43和E-钙粘蛋白的表达。将连接蛋白43基因转染肺癌细胞LH 7。采用免疫荧光染色和蛋白质印迹法检测连接蛋白43和E-钙粘蛋白的蛋白定位和水平。流式细胞仪和MTT法检测肺癌细胞的细胞周期和增殖。我们发现连接蛋白43和E-钙粘蛋白的表达降低与NSCLC的低分化、晚期TNM分期和淋巴结转移显著相关。Cx43和E-cadherin的表达呈显著正相关。连接蛋白43的过度表达显著诱导E-钙粘蛋白的表达。此外，连接蛋白43转染的LH 7细胞表现出显著降低的细胞增殖。G1期细胞比例增加，S期和G2期细胞明显减少。我们的结论是，同时减少连接蛋白43和E-钙粘蛋白可能有助于肺癌的发展。缝隙连接蛋白43可诱导E-cadherin表达，抑制肺癌细胞增殖和发展。
The interaction of connexin 43 and E-cadherin may play an important role in carcinogenesis and malignant behaviour of tumours. In this study, we examined the relationship between connexin 43 and E-cadherin in human non-small cell lung cancers (NSCLC). Expression levels of connexin 43 and E-cadherin were examined in 107 NSCLC specimens by immunohistochemistry. The connexin 43 gene was transfected into lung cancer LH7 cells. The protein localizations and levels of connexin 43 and E-cadherin were detected using immunofluorescence staining and western blot. Cell cycle and proliferation of lung cancer cells were examined using flow cytometry and MTT. We found that reduced expression of both connexin 43 and E-cadherin significantly correlated to poor differentiation, advanced TNM stage, and lymph note metastasis of NSCLCs. Connexin 43 and E-cadherin expression significantly correlated with each other. Over-expression of connexin 43 significantly induced E-cadherin expression. Moreover, connexin 43-transfected LH7 cells showed significantly decreased cell proliferation. The percentage of cells in G1 phase increased, while the number of cells in S and G2 phases significantly decreased. We concluded that concurrent reduction of connexin 43 and E-cadherin may contribute to the development of lung cancer. Connexin 43 may induce E-cadherin expression and inhibit cell proliferation and progression of lung cancer.