Viral Expression Directs the Fate of B Cells in Bovine Leukemia Virus-Infected Sheep
Viral Expression Directs the Fate of B Cells in Bovine Leukemia Virus-Infected Sheep
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DOI：
10.1128/jvi.05718-11
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发表时间：
2012-01-01
影响因子：
5.4
通讯作者：
Willems, Luc
中科院分区：
文献类型：
--
作者：
Florins, Arnaud;de Brogniez, Alix;Willems, Luc
据信宿主免疫应答严格控制δ逆转录病毒如人T淋巴细胞病毒1型（HTLV-1）和牛白血病病毒（BLV）的病毒复制。然而，这一假设尚未在体内得到明确证实。为了进一步评估BLV模型中免疫应答的重要性，我们研究了瞬时诱导病毒表达的细胞的命运。使用双荧光染料标记方法，我们表明，离体诱导病毒表达诱导体内B细胞的死亡率较高。此外，这些动物的环孢菌素治疗表明，需要有效的免疫应答来控制病毒表达细胞。
The host immune response is believed to tightly control viral replication of deltaretroviruses such as human T-lymphotropic virus type 1 (HTLV-1) and bovine leukemia virus (BLV). However, this assumption has not been definitely proven in vivo. In order to further evaluate the importance of the immune response in the BLV model, we studied the fate of cells in which viral expression was transiently induced. Using a dual fluorochrome labeling approach, we showed that ex vivo induction of viral expression induces higher death rates of B cells in vivo. Furthermore, cyclosporine treatment of these animals indicated that an efficient immune response is required to control virus-expressing cells.