Unbiased molecular analysis of T cell receptor expression using template-switch anchored RT-PCR.
Unbiased molecular analysis of T cell receptor expression using template-switch anchored RT-PCR.
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DOI：
10.1002/0471142735.im1033s94
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发表时间：
2011-08
影响因子：
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通讯作者：
Douek, Daniel C
中科院分区：
文献类型：
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作者：
Quigley, Maire F;Almeida, Jorge R;Price, David A;Douek, Daniel C
详细了解记忆和效应T细胞池中指导克隆选择的原则，对于进一步了解影响T细胞介导的有效免疫的因素至关重要，并对合理设计疫苗和免疫疗法具有直接意义。该单元提供了在任何定义的T细胞群中对所有表达的T细胞受体（TCR）基因产物进行无偏定量和表征的方法。该方法基于模板开关锚定的逆转录聚合酶链反应（RT - PCR），并针对直接离体分离的抗原特异性T细胞的分析进行了优化。Protoc。Immunol 94:10.33.1 10.33.16应承担的。©2011 by John Wiley & Sons, Inc。
A detailed knowledge of the principles that guide clonal selection within the memory and effector T cell pools is essential to further our understanding of the factors that influence effective T cell‐mediated immunity and has direct implications for the rational design of vaccines and immunotherapies. This unit provides methods for the unbiased quantification and characterization of all expressed T cell receptor (TCR) gene products within any defined T cell population. The approach is based on a template‐switch anchored reverse transcription–polymerase chain reaction (RT‐PCR) and is optimized for the analysis of antigen‐specific T cells isolated directly ex vivo.Curr. Protoc. Immunol. 94:10.33.1‐10.33.16. © 2011 by John Wiley & Sons, Inc.