Method for the construction and use of carbon fiber multibarrel electrodes for deep brain recordings in the alert animal
Method for the construction and use of carbon fiber multibarrel electrodes for deep brain recordings in the alert animal
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DOI：
10.1016/j.jneumeth.2008.12.009
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发表时间：
2009-04-15
影响因子：
3
通讯作者：
Blazquez, Pablo M.
中科院分区：
文献类型：
--
作者：
Inagaki, Keiichiro;Heiney, Shane A.;Blazquez, Pablo M.
Microiontophoresis of neuroactive substances during single unit recording in awake behaving animals can significantly advance our understanding of neural circuit function. Here, we present a detailed description of a method for constructing carbon fiber multibarrel electrodes suitable for delivering drugs while simultaneously recording single unit activity from deep structures, including brainstem nuclei and the cerebellum, in the awake behaving primate. We provide data that should aid in minimizing barrel resistance and the time required to fill long, thin multibarrel electrodes with solutions. We also show successful single unit recording from a variety of areas in the awake squirrel monkey central nervous system, including the vestibular nuclei, Interstitial Nucleus of Cajal, and the cerebellum. Our descriptions and data should be useful for investigators wishing to perform single unit recordings during microiontophoresis of neuroactive substances, particularly in deep structures of animals with chronically implanted recording chambers. (C) 2008 Elsevier B.V. All rights reserved.
