Posttranslational Modification of Pili upon Cell Contact Triggers N. meningitidis Dissemination
Posttranslational Modification of Pili upon Cell Contact Triggers N. meningitidis Dissemination
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DOI：
10.1126/science.1200729
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发表时间：
2011-02-11
期刊：
影响因子：
56.9
通讯作者：
Dumenil, Guillaume
中科院分区：
文献类型：
--
作者：
Chamot-Rooke, Julia;Mikaty, Guillain;Dumenil, Guillaume
革兰氏阴性杆菌脑膜炎奈瑟菌在10%至30%的人口中无症状地定植于喉咙，但喉咙定植也可作为进入血液（败血症）然后进入大脑（脑膜炎）的入口。定植是由被称为IV型菌毛的丝状细胞器介导的，它允许与宿主细胞相关的细菌聚集体的形成。我们发现与宿主细胞接触的脑膜炎奈瑟菌的增殖增加了一种细菌基因的转录，这种基因编码一种将磷酸甘油添加到IV型菌毛上的转移酶。这种不寻常的翻译后修饰特异性地释放了细菌之间依赖于IV型毛的接触。反过来，这种受调节的分离过程允许细菌繁殖到新的定植位点，并跨越上皮迁移，这是传播和侵袭性疾病的先决条件。
The Gram-negative bacterium Neisseria meningitidis asymptomatically colonizes the throat of 10 to 30% of the human population, but throat colonization can also act as the port of entry to the blood (septicemia) and then the brain (meningitis). Colonization is mediated by filamentous organelles referred to as type IV pili, which allow the formation of bacterial aggregates associated with host cells. We found that proliferation of N. meningitidis in contact with host cells increased the transcription of a bacterial gene encoding a transferase that adds phosphoglycerol onto type IV pili. This unusual posttranslational modification specifically released type IV pili-dependent contacts between bacteria. In turn, this regulated detachment process allowed propagation of the bacterium to new colonization sites and also migration across the epithelium, a prerequisite for dissemination and invasive disease.