Increased expression of interleukin 17 in inflammatory bowel disease
Increased expression of interleukin 17 in inflammatory bowel disease
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DOI：
10.1136/gut.52.1.65
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发表时间：
2003-01-01
期刊：
影响因子：
24.5
通讯作者：
Fujiyama, Y
中科院分区：
文献类型：
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作者：
Fujino, S;Andoh, A;Fujiyama, Y
背景和目的：白细胞介素 (IL) 17 是一种具有强烈促炎活性的细胞因子。在这项研究中，我们评估了炎症性肠病（IBD）患者发炎粘膜和血清中IL-17表达的变化。方法：通过内窥镜或手术从溃疡性结肠炎（UC）（n=20）、克罗恩病（CD）（n=20）、感染性结肠炎（n=5）、缺血性结肠炎（n=8）和正常结直肠组织（n=15）患者中获取组织样本。通过标准免疫组织化学程序评估 IL-17 表达。通过ELISA测定血清IL-17水平。采用逆转录聚合酶链式反应分析IL-17 mRNA表达。结果：正常结肠粘膜、感染性结肠炎、缺血性结肠炎样本中未检测到IL-17表达。在活动性UC和CD患者的发炎粘膜中，在CD3(+)T细胞或CD68(+)单核细胞/巨噬细胞中清楚地检测到IL-17表达。与不活动的患者相比，活动性UC和CD患者的IL-17(+)细胞平均数量显着增加。在正常粘膜中未检测到 IL-17 mRNA 表达，但在活动性 UC 和 CD 患者的粘膜中可检测到。正常人、感染性结肠炎或缺血性结肠炎患者的血清中未检测到IL-17，但IBD患者的IL-17水平显着升高。结论：1131)患者的粘膜和血清中IL-17表达升高。 IBD 中 IL-17 的表达可能与肠粘膜免疫和炎症反应的改变有关。
Background and aim: Interleukin (IL) 17 is a cytokine which exerts strong proinflammatory activities. In this study we evaluated changes in IL-17 expression in the inflamed mucosa and in the serum of patients with inflammatory bowel disease (IBD).Methods: Tissue samples were obtained endoscopically or surgically from patients with ulcerative colitis (UC) (n=20), Crohn's disease (CD) (n=20), infectious colitis (n=5), ischaemic colitis (n=8), and normal colorectal tissues (n=15). IL-17 expression was evaluated by a standard immunohistochemical procedure. Serum IL-17 levels were determined by ELISA. IL-17 mRNA expression was analysed by reverse transcriptase-polymerase chain reaction.Results: IL-17 expression was not detected in samples from normal colonic mucosa, infectious colitis, or ischaemic colitis. In the inflamed mucosa of active UC and CD patients, IL-17 expression was clearly detectable in CD3(+) T cells or CD68(+) monocytes/macrophages. The average number of IL-17(+) cells was significantly increased in active UC and CD patients compared with inactive patients. IL-17 mRNA expression was not detected in normal mucosa but was detectable in the mucosa from active UC and CD patients. IL-17 was not detected in the sera from normal individuals, infectious colitis, or ischaemic colitis patients but IL-17 levels were significantly elevated in IBD patients.Conclusions: IL-17 expression in the mucosa and serum was increased in 1131) patients. It is likely that IL-17 expression in IBD may be associated with altered immune and inflammatory responses in the intestinal mucosa.