Overexpression of Anti-mullerian Hormone Gene in vivo Affects Gonad Sex Differentiation in Undifferentiated Orange-Spotted Groupers (Epinephelus coioides)
Overexpression of Anti-mullerian Hormone Gene in vivo Affects Gonad Sex Differentiation in Undifferentiated Orange-Spotted Groupers (Epinephelus coioides)
复制标题
体内抗苗勒管激素基因过表达影响未分化橙斑石斑鱼（斜带石斑鱼）性腺性别分化
DOI：
10.3389/fendo.2019.00210
复制
发表时间：
2019-04-05
影响因子：
5.2
通讯作者：
Lin, Haoran
中科院分区：
文献类型：
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作者：
Han, Yulong;Zhao, Mi;Lin, Haoran
硬骨鱼的性别分化是对性别决定信号的反应，这些信号诱导性腺发育为卵巢或精巢。然而，鱼类的性别分化机制是多种多样的，在性别转换鱼类性腺分化的信息仍然有限。斜带石斑鱼是一种雌雄同体的鱼类，是研究脊椎动物性腺分化的理想模型。在本研究中，转录组数据显示，在性腺中amh和amhrll的表达水平在性别分化过程中增加。在此基础上，研究了过量表达抗苗勒管激素（Amh）对石斑鱼性腺发育的影响。雌性相关基因的表达水平和血清17 β-雌二醇水平降低，而雄性相关基因的表达和血清11-酮睾酮水平在喂食amh质粒的鱼中增加。AMH基因的过表达也促进了未分化石斑鱼精原细胞的增殖，并诱导了雄性性腺的发育，但这种雄性倾向是在雌性分化之前发生的。总之，这些结果表明，AMH质粒喂养AMH过表达诱导未分化石斑鱼雄性性腺发育。
Sex differentiation in teleost fishes occurs in response to sex determination signals, which induce the gonad to develop as either an ovary or testis. However, sex differentiation mechanisms in fishes are diverse, and information on gonad differentiation in sex changing fishes remains limited. The orange-spotted grouper (Epinephelus coioides) is a protogynous hermaphroditic fish that provides an ideal model for investigating gonad differentiation in vertebrates. In this study, Transcriptome data showed that expression levels of amh and amhrll in gonads were increased during sex differentiation. Then we investigated the effect of overexpression anti-Mullerian hormone (Amh) on gonad development in juvenile orange-spotted groupers. Expression levels of female-related genes and serum 17 beta-estradiol levels were decreased, while expression of male-related genes and serum 11-ketotestosterone levels were increased in fish fed with amh-plasmid. Overexpression of Amh was also promoted the spermatogonia proliferation and induced the development of male gonads in undifferentiated orange-spotted groupers, but that this male tendency was preceded by female differentiation. In summary, these results illustrated that Amh overexpression by amh-plasmid feeding induced male gonad development in undifferentiated groupers.