NEUTROPHIL MIGRATION INTO THE BOVINE UTERINE LUMEN FOLLOWING INTRAUTERINE INOCULATION WITH KILLED HAEMOPHILUS-SOMNUS
NEUTROPHIL MIGRATION INTO THE BOVINE UTERINE LUMEN FOLLOWING INTRAUTERINE INOCULATION WITH KILLED HAEMOPHILUS-SOMNUS
复制标题
DOI：
10.1530/jrf.0.0930341
复制
发表时间：
1991-11-01
期刊：
影响因子：
--
通讯作者：
WIDDERS, PR
中科院分区：
文献类型：
--
作者：
BUTT, BM;SENGER, PL;WIDDERS, PR
测量了死细菌宫内沉积后牛子宫冲洗液中的多形核中性粒细胞（PMN），并确定了发情期间免疫状态对 PMN 流入子宫腔的影响。使用来自睡眠嗜血杆菌的 270 kDa 外膜蛋白 (omp-270) 对荷斯坦小母牛进行免疫。在发情期间，免疫后的小母牛 (n = 21) 接受宫内接种，其中包含含有 omp-270 (n = 7) 的睡眠嗜血菌同源菌株的热灭活悬浮液、缺乏 omp-270 的睡眠嗜血菌异源菌株 (n = 7) 或磷酸盐缓冲盐水 (n = 7)。另外五头小母牛用延长的牛精液进行授精。在接种前（t0）和接种后 6、24、48、72、96 和 120 小时通过盐水灌洗收集子宫内容物。接种精液的小母牛仅在 t120 时进行灌洗。所有组均经历 PMN 浸润，在接种后 6 小时达到峰值，此后趋于下降。治疗组之间没有观察到差异，这表明细菌接种和免疫状态在引起 PMN 积液方面都不如冲洗程序本身那么重要。
Polymorphonuclear neutrophils (PMN) in bovine uterine flushings following intrauterine deposition of killed bacteria were measured and the effect of immune status on the influx of PMN into the uterine lumen during oestrus was determined. Holstein heifers were immunized with a 270-kDa outer-membrane protein (omp-270) from Haemophilus somnus. During oestrus, immunized heifers (n = 21) received an intrauterine inoculum of either a heat-killed suspension of a homologous strain of H. somnus containing omp-270 (n = 7), a heterologous strain of H. somnus lacking omp-270 (n = 7), or phosphate-buffered saline (n = 7). Five additional heifers were inseminated with extended bovine semen. Uterine contents were collected in saline lavage immediately before inoculation (t0) and at 6, 24, 48, 72, 96, and 120 h after inoculation. The semen-inoculated heifers were lavaged only at t120. All groups experienced PMN infiltration which peaked 6 h after inoculation and tended to decline thereafter. Differences were not observed between treatment groups, indicating that neither bacterial inoculation nor immune status was as important in eliciting PMN effusion as the flushing procedure itself.