Genetic characterization of four native Italian shepherd dog breeds and analysis of their relationship to cosmopolitan dog breeds using microsatellite markers
Genetic characterization of four native Italian shepherd dog breeds and analysis of their relationship to cosmopolitan dog breeds using microsatellite markers
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DOI：
10.1017/s1751731115001561
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发表时间：
2015-12-01
期刊：
影响因子：
3.6
通讯作者：
Polli, M.
中科院分区：
文献类型：
--
作者：
Bigi, D.;Marelli, S. P.;Polli, M.
到目前为止，对意大利本土犬种遗传多样性的研究很少。在这项研究中，我们的目的是估计和比较四个本地意大利牧羊犬品种的遗传多样性：Maremma，Bergamasco，Lupino del Gigante和Oropa牧羊犬。因此，一些在意大利广泛饲养了很长时间的世界性犬种也被考虑用于检查这些犬种对此处考虑的意大利本土犬种的可能影响。共212个人，属于10个不同的狗品种，采样和基因型使用18个常染色体微卫星位点。从品种多样性、品种关系和群体结构等方面分析了这些品种的遗传多样性。在这项研究中考虑的10个品种明显的遗传分化彼此，无论目前的人口规模和单独的育种历史的开始。遗传多样性水平解释了总遗传变异的20%。这里发现的HE水平与其他研究发现的一致。意大利本土犬种的遗传多样性普遍较高，与历史悠久、定义明确的国际犬种相比。由于边境牧羊犬似乎更接近意大利品种比其他世界性的牧羊犬在这里考虑，可能利用这个品种，以提高工作性能，在意大利传统的工作牧羊犬不能忽视。这里发现的数据和信息可以用于组织保护计划，以减少近亲繁殖，并尽量减少遗传变异的损失。
Very little research into genetic diversity of Italian native dog breeds has been carried out so far. In this study we aimed to estimate and compare the genetic diversity of four native Italian shepherd dog breeds: the Maremma, Bergamasco, Lupino del Gigante and Oropa shepherds. Therefore, some cosmopolitan dog breeds, which have been widely raised in Italy for a long time past, have also been considered to check possible influence of these dog populations on the Italian autochthonous breeds considered here. A total of 212 individuals, belonging to 10 different dog breeds, were sampled and genotyped using 18 autosomal microsatellite loci. We analyzed the genetic diversity of these breeds, within breed diversity, breed relationship and population structure. The 10 breeds considered in this study were clearly genetically differentiated from each other, regardless of current population sizes and the onset of separate breeding history. The level of genetic diversity explained 20% of the total genetic variation. The level of HE found here is in agreement with that found by other studies. The native Italian breeds showed generally higher genetic diversity compared with the long established, well-defined cosmopolitan dog breeds. As the Border Collie seems closer to the Italian breeds than the other cosmopolitan shepherd dogs considered here, a possible utilization of this breed to improve working performance in Italian traditional working shepherd dogs cannot be ignored. The data and information found here can be utilized in the organization of conservation programs planned to reduce inbreeding and to minimize loss of genetic variability.