Inhalation anesthetics induce apoptosis in normal peripheral lymphocytes in vitro
Inhalation anesthetics induce apoptosis in normal peripheral lymphocytes in vitro
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DOI：
10.1097/00000542-200112000-00028
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发表时间：
2001-12-01
期刊：
影响因子：
8.8
通讯作者：
Hashimoto, Y
中科院分区：
文献类型：
--
作者：
Matsuoka, H;Kurosawa, S;Hashimoto, Y
背景：作者推测吸入麻醉剂诱导淋巴细胞凋亡可能是围手术期淋巴细胞减少的部分原因。因此，他们评估了七氟醚和异氟醚是否会诱导正常外周血淋巴细胞凋亡。方法：将正常外周血单个核细胞暴露于七氟醚和异氟醚中，分别于暴露24 h（0.5、1.0、1.5 mM）和暴露6、12、24 h （1-5 mM）后，采用Annexin v -荧光素异硫氰酸酯-7-氨基放线菌素D流式细胞术检测细胞凋亡百分比。暴露24 li （1.5 mM）后，测定具有caspase 3样活性的淋巴细胞百分比。结果:凋亡的百分比lymhocytes增加剂量依赖性的方式(控制:5.1 + / - 1.4%,七氟醚:7.3 + / - 1.3%(0.5毫米),9.1 + / - 1.5%(1.0毫米),12.6 + / - 2.1%(1.5毫米),异氟烷:7.5 + / - 1.6%(0.5毫米),10.5 + / - 1.5%(1.0毫米),16.3 + / - 2.70%[1 - 5毫米])经过24小时的暴露和时间的方式(控制:1.2 + / - 0.4%(6小时),3.4 + / - 0.7% [12 h], 5.6 + / - 1.2%(24小时),七氟醚:1.8 + / - 0.4%(6小时),6.4 + / - 1.2% [12 h], 11.3 + / - 2.2%(24小时);异氟烷：2.6 +/- 0.5% [6 h], 8.8 +/- 1.5% [12 h],16.0 +/- 1.9% [24 h])，具有caspase 3-like活性的淋巴细胞百分比增加（对照组：10.0 +/- 1.1%，七氟烷：13.8 +/- 1.2%，异氟烷：17.0 +/- 1.3%）。七氟醚和异氟醚均可诱导细胞凋亡；体外外周淋巴细胞的剂量依赖性和时间依赖性。
Background The authors hypothesized that perioperative lymphocytopenia is partially caused by apoptosis of lymphocytes Induced by Inhalation anesthetics. Therefore, they evaluated whether sevoflurane and isoflurane induce apoptosis of normal peripheral lymphocytes.Methods: Normal peripheral blood mononuclear cells were exposed to sevoflurane and isoflurane, and the percentages of apoptotic lymphocytes was measured by Annexin V-fluorescein isothiocyanate-7-amino actinomycin D flow cytometry after 24 h of exposure (0.5, 1.0, and 1.5 mM) and after 6, 12, and 24 h of exposure (1-5 mm). The percentages of lymphocytes with caspase 3-like activity were also measured after 24 li of exposure (1.5 mM).Results: The percentages of apoptotic lymhocytes were increased in a dose-dependent manner (controls: 5.1 +/- 1.4%; sevoflurane: 7.3 +/- 1.3% [0.5 mM], 9.1 +/- 1.5% [1.0 mM], 12.6 +/- 2.1% [1.5 mM]; isoflurane: 7.5 +/- 1.6% [0.5 mM], 10.5 +/- 1.5% [1.0 mM], 16.3 +/- 2.70% [1-5 mM]) after 24 h of exposure and in a time-dependent manner (controls: 1.2 +/- 0.4% [6 h], 3.4 +/- 0.7% [12 h], 5.6 +/- 1.2% [24 h]; sevoflurane: 1.8 +/- 0.4% [6 h], 6.4 +/- 1.2% [12 h], 11.3 +/- 2.2% [24 h]; isoflurane: 2.6 +/- 0.5% [6 h], 8.8 +/- 1.5% [12 h],16.0 +/- 1.9% [24 h]) at the concentration of 1.5 mM. The percentages of lymphocytes with caspase 3-like activity were increased (controls: 10.0 +/- 1.1%; sevoflurane: 13.8 +/- 1.2%; isoflurane: 17.0 +/- 1.3%).Conclusions. Both sevofluraine and isoflurane Induced apoptosis; in peripheral lymphocytes in dose-dependent and timedependent manners in vitro.