Bromodomain-containing factor GTE4 regulates Arabidopsis immune response.
Bromodomain-containing factor GTE4 regulates Arabidopsis immune response.
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DOI：
10.1186/s12915-022-01454-5
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发表时间：
2022-11-13
期刊：
影响因子：
5.4
通讯作者：
Chen, Xiangsong
中科院分区：
文献类型：
--
作者：
Zhou, Qin;Sun, Yingnan;Zhao, Xiwang;Yu, Yue;Cheng, Weijia;Lu, Li;Chu, Zhaohui;Chen, Xiangsong
植物不断受到来自环境病原体的生物胁迫的挑战，精确调节防御反应对于植物的生存至关重要。防御系统需要大量的能量和资源，损害植物生长，控制转录调控的植物激素在建立对病原体的防御反应和生长之间的适当平衡中起着至关重要的作用。调节基因转录的染色质调节因子广泛参与调节胁迫应答基因。然而，哪些染色质因子参与协调植物激素信号和免疫反应，以及它们的功能机制仍然不清楚。在这里，我们确定了含溴结构域的蛋白GTE 4在负调控拟南芥防御反应中的作用。GTE 4主要在注射假单胞菌感染后作为基因表达的激活剂发挥作用。全基因组GTE 4占有率分析表明，GTE 4倾向于结合活性基因，包括核糖体生物发生相关基因，并在病原体感染期间维持其高表达水平。然而，GTE 4也能够抑制基因表达。GTE 4结合并抑制茉莉酸生物合成基因OPR 3。GTE 4的破坏导致茉莉酸（JA）的过度积累和增强的JA响应基因表达。出乎意料的是，gte 4突变体中积累的JA含量与JA介导的免疫防御基因的下调和水杨酸（SA）介导的免疫防御基因的上调相结合，并增强对假单胞菌的抗性，可能通过非经典途径。总的来说，我们确定了一个新的作用，染色质因子GTE 4作为植物免疫反应的负调节剂，通过抑制JA生物合成，这反过来又noncanonically激活防御系统对假单胞菌。这些发现提供了新的知识，在防御反应过程中的植物激素信号的颜色调节。在线版本包含补充材料，可通过10.1186/s12915-022-01454-5获得。
Plants are continuously challenged with biotic stress from environmental pathogens, and precise regulation of defense responses is critical for plant survival. Defense systems require considerable amounts of energy and resources, impairing plant growth, and plant hormones controlling transcriptional regulation play essential roles in establishing the appropriate balance between defense response to pathogens and growth. Chromatin regulators modulating gene transcription are broadly involved in regulating stress-responsive genes. However, which chromatin factors are involved in coordinating hormone signaling and immune responses in plants, and their functional mechanisms, remains unclear. Here, we identified a role of bromodomain-containing protein GTE4 in negatively regulating defense responses in Arabidopsis thaliana. GTE4 mainly functions as activator of gene expression upon infection with Pseudomonas syringe. Genome-wide profiling of GTE4 occupancy shows that GTE4 tends to bind to active genes, including ribosome biogenesis related genes and maintains their high expression levels during pathogen infection. However, GTE4 is also able to repress gene expression. GTE4 binds to and represses jasmonate biosynthesis gene OPR3. Disruption of GTE4 results in overaccumulation of jasmonic acid (JA) and enhanced JA-responsive gene expression. Unexpectedly, over-accumulated JA content in gte4 mutant is coupled with downregulation of JA-mediated immune defense genes and upregulation of salicylic acid (SA)-mediated immune defense genes, and enhanced resistance to Pseudomonas, likely through a noncanonical pathway. Overall, we identified a new role of the chromatin factor GTE4 as negative regulator of plant immune response through inhibition of JA biosynthesis, which in turn noncanonically activates the defense system against Pseudomonas. These findings provide new knowledge of chromatic regulation of plant hormone signaling during defense responses. The online version contains supplementary material available at 10.1186/s12915-022-01454-5.
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影响因子：
7.2
作者：
Duque, P;Chua, NH
通讯作者：
Chua, NH
影响因子：
5.6
作者：
Kimberlin A;Holtsclaw RE;Koo AJ
通讯作者：
Koo AJ
影响因子：
4.5
作者：
Gong F;Chiu LY;Miller KM
通讯作者：
Miller KM
影响因子：
16.6
作者：
Liu, Lijing;Sonbol, Fathi-Mohamed;Huot, Bethany;Gu, Yangnan;Withers, John;Mwimba, Musoki;Yao, Jian;He, Sheng Yang;Dong, Xinnian
通讯作者：
Dong, Xinnian
影响因子：
4.1
作者：
Hofr, Ctirad;Sultesova, Pavla;Fajkus, Jiri
通讯作者：
Fajkus, Jiri