The decapeptide CMS001 enhances swimming endurance in mice
The decapeptide CMS001 enhances swimming endurance in mice
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DOI：
10.1016/j.peptides.2008.03.004
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发表时间：
2008-07-01
期刊：
影响因子：
3
通讯作者：
Yao, Zhi
中科院分区：
文献类型：
--
作者：
Wang, Li;Zhang, Hua-Li;Yao, Zhi
肽类化合物具有吸收快、能量低、活性高等优点，在生物应用中具有蛋白质所不能比拟的优势。一些生物活性肽已被开发用于运动相关疾病的管理。本实验研究了从猪脾脏中分离得到的十肽CMS 001（Pro-Thr-Thr-Lys-Thr-Tyr-Phe-Pro-His-Phe）是否具有抗疲劳作用。给雄性Balb/c小鼠施用CMS 001（20 μ g/（kg d）（-1）或5 μ g/（kg d）（-1），持续30天，腹膜内注射），并在力竭游泳时间任务中进行测试。为了研究CMS 001抗疲劳作用的机制，我们分析了肝糖原储存、血尿素氮（BUN）水平、乳酸水平、超微结构完整性以及自由基代谢物和抗氧化酶的水平。相对于仅注射盐水的小鼠，CMS 001处理延长了力竭游泳时间，增加了肝糖原水平，降低了BUN水平，并减少了血液中乳酸的积累。对CMS 001处理的小鼠和对照小鼠的骨骼肌和心肌中的线粒体和肌浆网的超微结构的检查显示，CMS 001可以减少由力竭游泳挑战引起的对心脏和骨骼肌的损伤，使得肽处理组中的大多数组织标本的结构是正常的。此外，急性运动后的自由基分析表明，CMS 001治疗降低丙二醛（MDA）和增加超氧化物歧化酶（SOD）水平。目前的研究结果表明，脾源肽CMS 001对小鼠具有抗疲劳作用，并进一步表明其机制可能涉及减少肌肉组织中的组织损伤自由基。(C)2008年爱思唯尔公司All rights reserved.
Now peptides achieve distinct advantages over protein in biological application because of its quick and easy absorption, low power, and high activity. Some bioactive peptides had been developed to be used in the management of exercise-related disorders. In this study, we investigated whether the decapeptide CMS001 (Pro -Thr-Thr-Lys-Thr-Tyr-Phe-Pro-His-Phe) isolated from pig spleen had anti-fatigue effects. Male Balb/c mice were administered CMS001 (20 mu g/(kg d)(-1) or 5 mu g/(kg d)(-1) for 30 d, intraperitoneal injections) and tested in an exhaustive swim time task. In order to examine the mechanisms of CMS001 anti-fatigue effects, we analyzed liver glycogen stores, blood urea nitrogen (BUN) levels, lactic acid levels, ultrastructural integrity, and levels of both a free radical metabolite and an anti-oxidant enzyme. CMS001 treatment prolonged exhaustive swim time, increased liver glycogen levels, reduced BUN levels, and decreased accumulation of lactic acid in the blood, relative to mice injected with only saline. Examination of the ultrastructure of mitochondria and sarcoplasmic reticulum in skeletal and cardiac muscle of CMS001-treated and control mice revealed that CMS001 can reduce the damage to cardiac and skeletal muscle caused by an exhaustive swim challenge, such that the structure of most tissue specimens were normal in the peptide-treated group. Furthermore the free radical analysis after acute exercise indicated that CMS001 treatment decreased malondialdehyde (MDA) and increased superoxide dismutase (SOD) levels. The present findings indicate that the spleen-derived peptide CMS001 has anti-fatigue effects in mice, and further suggest that the mechanism may involve reduction of tissue damaging free radicals in muscle tissues. (C) 2008 Elsevier Inc. All rights reserved.