Development of normal mice from oocytes injected with freeze-dried spermatozoa
Development of normal mice from oocytes injected with freeze-dried spermatozoa
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DOI：
10.1038/nbt0798-639
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发表时间：
1998-07-01
影响因子：
46.9
通讯作者：
Yanagimachi, R
中科院分区：
文献类型：
--
作者：
Wakayama, T;Yanagimachi, R
冻干的老鼠精子在传统意义上都是静止的和死亡的。然而，当注入卵母细胞时，即使在干燥状态下保存三个月，它们的细胞核也能支持正常的胚胎发育。虽然这里报道的冷冻干燥方案还需要进一步改进，但结果表明，在室温下储存男性基因组是可能的。
Freeze-dried mouse spermatozoa are all motionless and dead in the conventional sense. When injected into oocytes, however, their nuclei can support normal embryonic development even after three month preservation in a dried state. Although the freeze-drying protocol reported here will need further improvement, the results suggest that it may be possible to store the male genomes at room temperature.