Expression of mRNA for matrix metalloproteinases and tissue inhibitors of metalloproteinases in periodontitis-affected human gingival tissue
Expression of mRNA for matrix metalloproteinases and tissue inhibitors of metalloproteinases in periodontitis-affected human gingival tissue
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DOI：
10.1016/0003-9969(95)00126-3
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发表时间：
1996-03-01
影响因子：
3
通讯作者：
Hara, K
中科院分区：
文献类型：
--
作者：
Kubota, T;Nomura, T;Hara, K
已知宿主对胶原酶[或基质金属蛋白酶-1]浓度升高的反应是通过优先表达金属蛋白酶组织抑制因子-L(TIMP-1)来克服牙周炎引起的组织破坏。为进一步阐明MMPs与TIMPs在牙周炎组织中的关系，采用逆转录-聚合酶链式反应技术检测了10例牙周炎患者和5例健康人牙周组织中MMPs、TIMP-1和TIMP-2的表达。病变组MMP1、MMP3、MMP3和TIMP1mRNA相对β-肌动蛋白的表达水平显著高于对照组(Mean+/-SE：病变组和健康组(%)：MMP1：0.26+/-0.05vs0.018+/-0.0040；MMP3：0.90+/-0.16vs0.063+/-0.016；MMP8：0.068+/-0.017 vs0.006+/-0.0010；TIMP-1的12.66+/-2.90比2.71+/-0.54；p<；0.01)。TIMP-2在两组间差异无统计学意义(1.79+/-0.33vs1.42+/-0.53；p>；0.05)。在炎症牙周组织中，由于基质金属蛋白酶-3是一种广谱基质金属蛋白酶，是潜伏性基质金属蛋白酶-1和-8的关键激活剂，因此炎症牙龈组织中基质金属蛋白酶-3的表达水平相对于基质金属蛋白酶-1和-8的表达水平的优先升高可能与组织破坏有关。因此，在牙周炎结缔组织破坏过程中，牙周组织中基质金属蛋白酶-1、-3和-8mRNA的表达增加可能是MMPs协同作用的原因。版权所有(C)1996爱思唯尔科学有限公司。
It is known that the host responds to an increased concentration of collagenase [or matrix metalloproteinase (MMP)-1] by preferentially expressing mRNA for the tissue inhibitor of metalloproteinase-l (TIMP-1) in order to overcome tissue destruction due to periodontitis. To further elucidate the relation between MMPs and TIMPs in periodontitis-affected tissues, the expression of mRNA for MMP-1, -3 and -8, and TIMP-1 and -2, in 10 gingival samples from patients and five from healthy individuals was assessed by reverse transcription-polymerase chain reaction. The diseased group showed significantly higher levels of MMP-1, -3, -8 and TIMP-1 mRNA relative to beta-actin than the control group (mean +/- SE: diseased vs healthy (%): 0.26 +/- 0.05 vs 0.018 +/- 0.0040 for MMP-1; 0.90 +/- 0.16 vs 0.063 +/- 0.016 for MMP-3; 0.068 +/- 0.017 vs 0.006 +/- 0.0010 for MMP-8; 12.66 +/- 2.90 vs 2.71 +/- 0.54 for TIMP-1; p < 0.01). TIMP-2 did not significantly differ between the two groups (1.79 +/- 0.33 vs 1.42 +/- 0.53; p > 0.05). The preferential increase in the level of MMP-3 mRNA relative to that of MMP-1 and -8 in inflamed gingiva would be relevant to tissue destruction because MMP-3 is a broad-spectrum MMP and a pivotal activator of latent MMP-1 and -8. Therefore, the overall increase in MMP-1, -3 and -8 mRNA in periodontitis-affected gingiva might account for a concerted action of MMPs during connective tissue destruction in periodontitis. Copyright (C) 1996 Elsevier Science Ltd.