Angiotensin-dependent gene expression in the developing rat kidney.
Angiotensin-dependent gene expression in the developing rat kidney.
复制标题
发育中的大鼠肾脏中血管紧张素依赖性基因的表达。
DOI：
10.1046/j.1523-1755.1998.06729.x
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Gomez,RA
中科院分区：
文献类型：
--
作者：
Hilgers,KF;Pentz,ES;Gomez,RA
血管紧张素依赖性基因在大鼠肾脏发育过程中的表达我们的目的是找出与血管紧张素II(Ang II)在肾脏发育过程中的生长效应有关的基因。经血管紧张素II受体阻滞剂氯沙坦治疗后，经Northern印迹法检测，转化生长因子β1、血小板衍生生长因子B、血管内皮生长因子和表皮生长因子的表达无明显变化(每组4~6只)。差异显示方法用于识别依赖Ang II表达的基因，产生的大多数是假阳性。我们发现了一个新的大鼠部分基因，命名为Ad.5，它与人类的孤儿受体有关。Ad.5以发育调节的方式表达，可能参与肾脏的发育和/或Ang II的营养作用。
Angiotensin-dependent gene expression in the developing rat kidney.We aimed to identify genes involved in the growth effects of angiotensin II (Ang II) during kidney development. In rats treated from birth with the Ang II type-1 receptor blocker losartan, expression of transforming growth factor β1 (TGF-β1), platelet-derived growth factor B (PDGF-B), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF), as measured by Northern blot, did not change significantly (N= 4 to 6 per group each). Differential display methods, used to identify genes with Ang II-dependent expression, produced mostly false positives. We identified one novel rat partial cDNA, termed AD.5, that is related to a human orphan receptor. AD.5 was expressed in a developmentally regulated pattern and may be involved in kidney development and/or the trophic actions of Ang II.