Magnetic relaxation measurement in immunoassay using high-transition-temperature superconducting quantum interference device system
Magnetic relaxation measurement in immunoassay using high-transition-temperature superconducting quantum interference device system
复制标题
DOI：
10.1063/1.2203390
复制
发表时间：
2006-06-15
影响因子：
3.2
通讯作者：
Hong, Chin-Yih
中科院分区：
文献类型：
--
作者：
Yang, H. C.;Yang, S. Y.;Hong, Chin-Yih
超导量子干涉器件（squid）由于其对磁通量的超高灵敏度，能够探测生物磁学信号。通过用磁性纳米颗粒标记生物靶标，一些研究小组已经表明，鱿鱼可以通过测量生物靶标的磁性来作为生物靶标的定量探针。在这项工作中，我们描述了一个高转变温度射频SQUID磁强计系统的设计和构建，用于测量标记亲和素的磁弛豫。我们还描述了磁性纳米颗粒包被生物素用于标记亲和素的合成。此外，还探讨了基于squid的亲和素磁标记免疫分析的规范。
Due to their ultrahigh sensitivity to magnetic flux, superconducting quantum interference devices (SQUIDs) are able to detect biomagnetic signals. By labeling biotargets with magnetic nanoparticles, several groups have shown that SQUIDs are promising as quantitative probes of biotargets by measuring their magnetic properties. In this work, we describe the design and construction of a high-transition-temperature radio-frequency SQUID magnetometer system for measuring the magnetic relaxation of labeled avidin. We also describe the synthesis of magnetic nanoparticles coated with biotin for use in labeling the avidin. Furthermore, the specifications of the SQUID-based magnetically labeled immunoassay of avidin are explored.