Chloroplast Translation Elongation Factor EF-Tu/SVR11 Is Involved in var2-Mediated Leaf Variegation and Leaf Development in Arabidopsis
Chloroplast Translation Elongation Factor EF-Tu/SVR11 Is Involved in var2-Mediated Leaf Variegation and Leaf Development in Arabidopsis
复制标题
叶绿体翻译伸长因子 EF-Tu/SVR11 参与 var2 介导的拟南芥叶片杂色和叶片发育
DOI：
10.3389/fpls.2019.00295
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发表时间：
2019-03-12
影响因子：
5.6
通讯作者：
Qi, Yafei
中科院分区：
文献类型：
--
作者：
Liu, Siyu;Zheng, Lu;Qi, Yafei
叶绿体是一种半自主的细胞器，在进化过程中保留了自己的基因组和基因表达装置，但受核基因组编码的蛋白质因子控制。为了分析FtsH介导的拟南芥叶绿体发育的遗传调控网络，已经鉴定了一组黄色杂色（var 2）的抑制突变体。在这项研究中，我们报告了另一个新的var 2抑制基因座，变异的供应商11（SVR 11），它编码一个假定的叶绿体定位的原核类型的翻译延伸因子EF-Tu的鉴定。SVR 11可能是叶绿体发育和植物存活所必需的。GUS活性显示SVR 11在幼叶组织、侧根和根尖中含量丰富。有趣的是，我们发现SVR 11和SVR 9共同调控叶片发育，包括叶缘发育和子叶脉序模式。这些发现加强了叶绿体翻译状态触发的逆行信号不仅调节叶绿体发育，而且调节叶发育的观点。
Chloroplasts are semiautonomous organelles, retaining their own genomes and gene expression apparatuses but controlled by nucleus genome encoded protein factors during evolution. To analyze the genetic regulatory network of FtsH-mediated chloroplast development in Arabidopsis, a set of suppressor mutants of yellow variegated (var2) have been identified. In this research, we reported the identification of another new var2 suppressor locus, SUPPRESSOR OF VARIEGATION11 (SVR11), which encodes a putative chloroplast-localized prokaryotic type translation elongation factor EF-Tu. SVR11 is likely essential to chloroplast development and plant survival. GUS activity reveals that SVR11 is abundant in the juvenile leaf tissue, lateral roots, and root tips. Interestingly, we found that SVR11 and SVR9 together regulate leaf development, including leaf margin development and cotyledon venation patterns. These findings reinforce the notion that chloroplast translation state triggers retrograde signals regulate not only chloroplast development but also leaf development.