A new and sensitive method for the quantification of HBV cccDNA by real-time PCR
A new and sensitive method for the quantification of HBV cccDNA by real-time PCR
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DOI：
10.1016/s0006-291x(02)00813-6
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发表时间：
2002-08-02
影响因子：
3.1
通讯作者：
Kung, HF
中科院分区：
文献类型：
--
作者：
He, ML;Wu, J;Kung, HF
B型肝炎病毒（HBV）共价闭合环状（ccc）DNA在肝细胞中的持续存在被认为是HBV抗病毒治疗完成后复发的主要原因。到目前为止，还没有灵敏的方法来定量感染的肝细胞中的cccDNA。我们设计了一组引物，用于特异性扩增HBV cccDNA的DNA片段，但不扩增病毒基因组DNA。当在定量实时PCR中使用100-10（7）拷贝的HBV cccDNA作为模板时，获得良好的线性范围。该方法不仅快速、经济、灵敏度高，而且可用于监测感染的人肝活检组织中的HBV cccDNA，并指导患者接受长期抗HBV治疗。(C)2002年爱思唯尔科学（美国）。All rights reserved.
The persistence of covalently closed circular (ccc) DNA of Hepatitis B virus (HBV) in liver cells is believed to be the major reason for relapse after completion of HBV antiviral therapy. Up to now, there is no sensitive method to quantify cccDNA in infected liver cells. We designed a set of primers to specifically amplify DNA fragments from HBV cccDNA but not from viral genomic DNA. A good linear range was obtained when 100-10(7) copies of HBV cccDNA were used as template in the quantitative real-time PCR. Not only is this method rapid, economical, highly sensitive, it can be used to monitor HBV cccDNA in infected human liver biopsies and to guide patients undergoing long-term anti-HBV therapy. (C) 2002 Elsevier Science (USA). All rights reserved.