Blood clot detection using magnetic nanoparticles.
Blood clot detection using magnetic nanoparticles.
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DOI：
10.1063/1.4977073
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发表时间：
2017-05
期刊：
影响因子：
1.6
通讯作者：
Weaver JB
中科院分区：
文献类型：
--
作者：
Khurshid H;Friedman B;Berwin B;Shi Y;Ness DB;Weaver JB
深静脉血栓形成（外周静脉中形成血栓）是一种非常严重、危及生命的疾病，在老年人中普遍存在。为了提供适当的治疗以提高存活率，尽早并在护理时发现血栓非常重要。我们探索了磁粒子光谱（MSB）通过适配体功能化磁性纳米颗粒与血凝块的特异性结合来检测血栓的能力。 MSB 使用纳米颗粒在交变磁场中产生的谐波来测量旋转自由度，从而测量纳米颗粒的束缚态。纳米颗粒的布朗旋转弛豫时间在结合时会增加 [A.M. Rauwerdink 和 J. B. Weaver，应用。物理。莱特。 96, 1(2010)]。因此，弛豫时间可用于表征纳米颗粒与血凝块中凝血酶的结合。对于较长的弛豫时间，达到饱和的方法是逐渐减少高次谐波和谐波比。与不含 ATP 的纳米颗粒相比，当样品介质中存在血凝块时，与抗凝血酶适体 (ATP) 缀合的纳米颗粒的谐波比随时间显着降低。此外，从样品介质中取出的血凝块产生了显着的 MSB 信号，表明纳米颗粒固定在血凝块上。我们的结果表明，MSB 可能是一种非常有用的非侵入性快速工具，可在护理点检测血栓，因此可以采用适当的治疗来降低深静脉血栓形成的固有风险。
Deep vein thrombosis, the development of blood clots in the peripheral veins, is a very serious, life threatening condition that is prevalent in the elderly. To deliver proper treatment that enhances the survival rate, it is very important to detect thrombi early and at the point of care. We explored the ability of magnetic particle spectroscopy (MSB) to detect thrombus via specific binding of aptamer functionalized magnetic nanoparticles with the blood clot. MSB uses the harmonics produced by nanoparticles in an alternating magnetic field to measure the rotational freedom and, therefore, the bound state of the nanoparticles. The nanoparticles’ relaxation time for Brownian rotation increases when bound [A.M. Rauwerdink and J. B. Weaver, Appl. Phys. Lett. 96, 1 (2010)]. The relaxation time can therefore be used to characterize the nanoparticle binding to thrombin in the blood clot. For longer relaxation times, the approach to saturation is more gradual reducing the higher harmonics and the harmonic ratio. The harmonic ratios of nanoparticles conjugated with anti-thrombin aptamers (ATP) decrease significantly over time with blood clot present in the sample medium, compared with nanoparticles without ATP. Moreover, the blood clot removed from the sample medium produced a significant MSB signal, indicating the nanoparticles are immobilized on the clot. Our results show that MSB could be a very useful non-invasive, quick tool to detect blood clots at the point of care so proper treatment can be used to reduce the risks inherent in deep vein thrombosis.