Circ11103 Interacts with miR-128/PPARGC1A to Regulate Milk Fat Metabolism in Dairy Cows
Circ11103 Interacts with miR-128/PPARGC1A to Regulate Milk Fat Metabolism in Dairy Cows
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Circ11103 与 miR-128/PPARGC1A 相互作用调节奶牛乳脂代谢
DOI：
10.1021/acs.jafc.0c07018
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发表时间：
2021-04-07
影响因子：
6.1
通讯作者：
Yang, Zhangping
中科院分区：
文献类型：
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作者：
Chen, Zhi;Lu, Qinyue;Yang, Zhangping
牛奶的品质与其乳成分密不可分，而脂肪酸含量是影响牛奶品质的关键因素。本研究通过高通量测序测定了干奶期和泌乳高峰期牛乳腺组织的 miRNA 和 mRNA 谱。总共筛选了 72 条 miRNA-mRNA 调控通路，包括 miR-128/PPARGC1A 调控通路。 miR-128可以直接靶向PPARGC1A并抑制其表达。此外，研究还观察到环状RNA circ11103的序列中存在miR-128结合位点，circ11103显着降低了miR-128的表达。 circ11103上调牛乳腺上皮细胞（BMEC）中的甘油三酯水平并增加不饱和脂肪酸的含量。然而，miR-128 降低了 BMEC 中的甘油三酯和胆固醇水平。本研究旨在分析circ11103对乳脂代谢的调节作用机制，为提高牛奶质量提供新的见解。
The quality of milk is inseparable from its milk components, and fatty acid content is a key factor affecting the quality of milk. In this study, the miRNA and mRNA profiles of the bovine mammary gland tissue during the dry period and the peak lactation period were determined through high-throughput sequencing. In total, 72 miRNA-mRNA regulatory pathways were screened, including miR-128/PPARGC1A regulatory pathways. miR-128 can directly target PPARGC1A and inhibit its expression. In addition, the study also observed that there was a miR-128 binding site in the sequence of the circular RNA circ11103, and circ11103 significantly reduced the expression of miR-128. circ11103 upregulated the triglyceride levels in bovine mammary epithelial cells (BMECs) and increased the contents of unsaturated fatty acids. However, miR-128 decreased triglyceride and cholesterol levels in BMECs. This study aims to analyze the mechanism governing the regulatory effect of circ11103 on milk fat metabolism, which provides new insights into improving milk quality.