Correction to the amino acid sequence of porcine motilin.
Correction to the amino acid sequence of porcine motilin.
复制标题
猪胃动素氨基酸序列的校正。
DOI：
10.1139/o74-002
复制
发表时间：
1974
期刊：
影响因子：
--
通讯作者：
J. Brown
中科院分区：
文献类型：
--
作者：
H. Schubert;J. Brown
天然猪胃动素的胰蛋白酶肽和用正亮氨酸取代蛋氨酸的合成类似物的电泳迁移率的比较揭示了天然材料中不存在酸性肽TR3。使用亮氨酸氨基肽酶和丹酰基-埃德曼降解对该肽进行的动力学研究表明，14 位存在谷氨酰胺，而不是之前报道的谷氨酸。这表明在早期制备天然猪胃动素时，谷氨酰胺发生了脱酰胺作用。
A comparison of the electrophoretic mobilities of the tryptic peptides of natural porcine motilin and a synthetic analogue with norleucine substituted for methionine revealed the absence of the acidic peptide TR3 of the natural material. Kinetic studies with leucine aminopeptidase and dansyl-Edman degradations on this peptide revealed the presence of glutamine at position 14 and not glutamic acid as previously reported. It is suggested that in the earlier preparation of natural porcine motilin deamidation of glutamine occurred.