Growth of B95-8 cells and expression of Epstein-Barr virus lytic phase in serum-free medium
Growth of B95-8 cells and expression of Epstein-Barr virus lytic phase in serum-free medium
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DOI：
10.1128/jvi.61.12.4033-4037.1987
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发表时间：
1987-12
影响因子：
5.4
通讯作者：
J. Shaw;R. Petit;K. Leung
中科院分区：
文献类型：
--
作者：
J. Shaw;R. Petit;K. Leung
选择Epstein-Barr病毒（EBV）转化的绢毛猴（Saguinus oedipus）细胞（B 95 -8）在具有减少的血清的培养基中生长，然后转移到由补充有胰岛素、转铁蛋白和硒的RPMI 1640组成的无血清培养基中。连续传代1年的无血清细胞的形态、生长速率和培养密度接近有血清生长的B 95 -8细胞。这些细胞表达病毒诱导的抗原，包括EBV相关的DNA聚合酶。暴露于EBV诱导剂，正丁酸和佛波醇12-肉豆蔻酸酯-13-乙酸酯的细胞产生转化病毒，其滴度与用血清生长的培养物的滴度相当。这些发现表明，血清既不是B 95 -8细胞生长所必需的，也不是这些细胞中EBV裂解期的诱导或完全表达所必需的。
Epstein-Barr virus (EBV)-transformed tamarin (Saguinus oedipus) cells (B95-8) were selected for growth in medium with reduced serum and then transferred to serum-free medium which consisted of RPMI 1640 supplemented with insulin, transferrin, and selenium. Serum-free cells in continuous passage for 1 year had a morphology, growth rate, and culture density which approached those of B95-8 cells grown with serum. The cells expressed virus-induced antigens, including the EBV-associated DNA polymerase. Cells exposed to EBV-inducing agents, n-butyric acid and phorbol 12-myristate-13-acetate, produced transforming virus with titers comparable to those of cultures grown with serum. These findings demonstrate that serum is neither required for the growth of B95-8 cells nor necessary for induction or full expression of the EBV lytic phase in these cells.