Salivary microbiota levels in relation to periodontal status, experience of caries and miswak use in Sudanese adults
Salivary microbiota levels in relation to periodontal status, experience of caries and miswak use in Sudanese adults
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DOI：
10.1034/j.1600-051x.2002.290505.x
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发表时间：
2002-05-01
影响因子：
6.7
通讯作者：
Ali, RW
中科院分区：
文献类型：
--
作者：
Darout, IA;Albandar, JM;Ali, RW
目的：本研究的目的是评估与牙周状况和龋齿经验有关的25种口腔细菌的唾液水平，并比较苏丹成年受试者中习惯性miswak和牙刷使用者之间这些细菌的水平。材料和方法：研究对象包括56名年龄范围为19-53岁（平均35.2岁）的个体，其中包括30名miswak和26名牙刷使用者。牙周状况和龋齿的存在进行了临床评估。收集所有受试者的全唾液，并通过使用全基因组DNA探针的棋盘DNA-DNA杂交方法评估唾液中25种选定细菌的水平。结果：高百分比的受试者具有唾液中几种细菌的可检测水平（大于或等于10（5）个细菌细胞）。12%-16%的受试者唾液中牙周炎相关细菌A水平较高（大于或等于10（6）个细胞）。actinomycetemcomitans、P. melaninogenica、P. intermedia、C.直肌和腐蚀性肠球菌，而只有两个（3.6%）和四个（7.1%）的受试者有高水平的牙龈卟啉单胞菌和F核，分别。所有或大多数细菌物种的水平没有显着差异，年龄组，性别或牙周状况。存在大于或等于10（5）L。唾液中的嗜酸性细菌细胞与高龋齿评分相关（p = 0.02）。A的水平明显较高。actinomycetemcomitans、P. melaninogenica、C. rectus、P. micros、V. parvula、S. mutans，S. anginosus、A. israelii、生痰梭菌和牙龈梭菌，而中间普氏杆菌、具核梭菌、S. sputigena、腐蚀艾美耳球虫（E corrodens）、L. acidophilus，S. sanguis，S. salivarius，S. oralis和S.结论：研究结果表明，miswak可能有选择性抑制唾液中的某些细菌，特别是几个口腔链球菌的水平的影响。这是第一份报告，棋盘DNA-DNA杂交方法可以用于评估唾液中的细菌类群的范围广泛的水平。
Objectives : The purpose of the present investigation was to assess the salivary levels of 25 oral bacteria in relation to periodontal status and experience of caries, and to compare the levels of these bacteria between habitual miswak and toothbrush users in adult Sudanese subjects.Material and methods: The study subjects consisted of 56 individuals with age range 19-53 years (mean 35.2 years) and included 30 miswak and 26 toothbrush users. The periodontal status and presence of dental caries were assessed clinically. Whole saliva was collected from all subjects, and the levels of 25 selected bacterial species in saliva were assessed by the checkerboard DNA-DNA hybridization method using whole genomic DNA probes.Results: A high percentage of the subjects had detectable levels (greater than or equal to 10(5) bacterial cells) of several bacterial species in saliva. Between 12% and 16% of the subjects showed high (greater than or equal to 10(6) cells) salivary levels of the periodontitis-associated bacteria A. actinomycetemcomitans, P. melaninogenica, P. intermedia, C. rectus and E corrodens, whereas only two (3.6%) and four (7.1%) subjects had high levels of P. gingivalis and F nucleatum, respectively There were no significant differences in the levels of all or most bacterial species by age group, gender or periodontal status. Presence of greater than or equal to 10(5) L. acidophilus bacterial cells in saliva was associated with high caries scores (p = 0.02). There were significantly higher levels of A. actinomycetemcomitans, P. melaninogenica, C rectus, P. micros, V. parvula, S. mutans, S. anginosus, A. israelii, C sputigena, and C gingivalis, and significantly lower levels of P. intermedia, F nucleatum, S. sputigena, E corrodens, L. acidophilus, S. sanguis, S. salivarius, S. oralis, and S. mitis in the miswak than in the toothbrush group.Conclusions : The findings suggest that miswak may have a selective inhibitory effect on the level of certain bacteria in saliva, particularly several oral streptococci species. This is the first report that the checkerboard DNA-DNA hybridization method can be useful for assessing the levels of a wide range of bacterial taxa in saliva.