Affibody-displaying bionanocapsules for specific drug delivery to HER2-expressing cancer cells
Affibody-displaying bionanocapsules for specific drug delivery to HER2-expressing cancer cells
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DOI：
10.1016/j.bmcl.2010.08.011
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发表时间：
2010-10-01
影响因子：
2.7
通讯作者：
Kondo, Akihiko
中科院分区：
文献类型：
--
作者：
Shishido, Takuya;Mieda, Hiroaki;Kondo, Akihiko
使用生物纳米胶囊（BNC）开发了一种新的HER 2靶向载体。生物纳米胶囊（BNC）是由B型肝炎病毒表面抗原的L蛋白组成的100 nm中空纳米颗粒。在BNC表面上遗传地展示HER 2的嵌合体（Z（HER 2）-BNC）。为了研究结合亲和力，将Z（HER 2）-BNC与癌细胞系SK-BR-3（HER 2阳性）和MDA-MB-231（HER 2阴性）孵育。为了分析HER 2靶向特异性，将Z（HER 2）-BNC或Z（WT）-BNC（不含抗体）与SK-BR-3和MDA-MB-231细胞一起孵育，通过时间推移和浓缩。对于包封分子（钙黄绿素）的递送，还通过与SK-BR-3细胞孵育将与脂质体混合的Z（HER 2）-BNC的荧光与Z（WT）-BNC和裸脂质体的荧光进行比较。结果，Z（HER 2）-BNC-脂质体复合物证明以高度特异性递送至HER 2表达细胞（SK-BR-3）。这表明基因工程化的BNC是有希望的癌症治疗载体。(C)2010爱思唯尔有限公司版权所有。
A novel HER2-targeted carrier was developed using bionanocapsules (BNCs). Bionanocapsules (BNCs) are 100-nm hollow nanoparticles composed of the L-protein of hepatitis B virus surface antigen. An affibody of HER2 was genetically displayed on the BNC surface (Z(HER2)-BNC). For the investigation of binding affinity, Z(HER2)-BNC was incubated with the cancer cell lines SK-BR-3 (HER2 positive), and MDA-MB-231 (HER2 negative). For analysis of HER2 targeting specificity, Z(HER2)-BNC or Z(WT)-BNC (without affibody) was incubated with both SK-BR-3 and MDA-MB-231 cells by time lapse and concentration. For the delivery of encapsulated molecules (calcein), fluorescence of Z(HER2)-BNC mixed with liposomes was also compared with that of Z(WT)-BNC and nude liposomes by incubation with SK-BR-3 cells. As a result, Z(HER2)-BNC-liposome complex demonstrated the delivery to HER2-expressing cells (SK-BR-3) with a high degree of specificity. This indicates that genetically engineered BNCs are promising carrier for cancer treatment. (C) 2010 Elsevier Ltd. All rights reserved.