Application of pressure assisted electrokinetic injection technique in the measurements of DNA oligonucleotides and their adducts using capillary electrophoresis-mass spectrometry
Application of pressure assisted electrokinetic injection technique in the measurements of DNA oligonucleotides and their adducts using capillary electrophoresis-mass spectrometry
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DOI：
10.1016/j.chroma.2007.02.071
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发表时间：
2007-05-04
影响因子：
4.1
通讯作者：
Zhu, Jiping
中科院分区：
文献类型：
--
作者：
Feng, Yong-Lai;Lian, Hongzhen;Zhu, Jiping
本文介绍了带负电荷的DNA寡核苷酸及其苯并[a]芘-7,8,9,10-四氢-7,8-二氢二醇- 9,10-环氧化物（BPDE）加合物的毛细管区带电泳（CZE）联用质谱（MS）系统，采用恒压辅助电动注射（PAEKI）在线富集技术进行鉴定和测定。在优化的PAEKI条件下，在90 s的PAEKI注射过程中，单链和双链寡核苷酸的在线样品浓度均可达到300-800倍。在扫描模式下，单四极杆质谱的检出限分别为0.01 ~ 0.03 RM和0.04 ~ 0.08 μ M。寡核苷酸在1 μ M处的相对标准偏差为7.6 ~ 15.8%。动态线性校准范围约为两个数量级。单四极杆质谱法可以在低微摩尔水平下获得较好的寡核苷酸和bpde寡核苷酸加合物的质谱，为DNA加合物的研究提供了一种有用的工具。(C) 2007 Elsevier B.V.版权所有
The identification and measurement of negatively charged DNA oligonucleotides and their benzo[a]pyrene-7,8,9,10-tetrahydro-7,8-dihydrodiol-9, 1 0-epoxide (BPDE) adducts by capillary zone electrophoresis (CZE) hyphenated mass spectrometry (MS) system using an on-line enrichment technique, the constant pressure assisted electrokinetic injection (PAEKI), is described here. With optimized PAEKI conditions, an on-line sample concentration power of 300-800 times could be reached for both single-stranded (ss) and double-stranded (ds) oligonucleotides during a 90-s PAEKI injection. The detection limits using single quadrupole MS in the scan mode were 0.01-0.03 RM for ss and 0.04-0.08 mu M for ds oligonucleotides, respectively. The relative standard deviations at 1 mu M of oligonucleotides were from 7.6 to 15.8%. A dynamic linear calibration range of about two orders of magnitude were observed. Good mass spectra of oligonucleotides and BPDE-oligonucleotide adducts at low micromolar levels could be obtained using single quadrupole MS which could be a helpful tool in DNA adducts studies. (C) 2007 Elsevier B.V. All rights reserved.