COOPERATIVE INTERACTIONS OF LFA-1 AND MAC-1 WITH INTERCELLULAR-ADHESION MOLECULE-1 IN FACILITATING ADHERENCE AND TRANSENDOTHELIAL MIGRATION OF HUMAN-NEUTROPHILS INVITRO
COOPERATIVE INTERACTIONS OF LFA-1 AND MAC-1 WITH INTERCELLULAR-ADHESION MOLECULE-1 IN FACILITATING ADHERENCE AND TRANSENDOTHELIAL MIGRATION OF HUMAN-NEUTROPHILS INVITRO
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DOI：
10.1172/jci114111
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发表时间：
1989-06-01
影响因子：
15.9
通讯作者：
ANDERSON, DC
中科院分区：
文献类型：
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作者：
SMITH, CW;MARLIN, SD;ANDERSON, DC
人中性粒细胞对人脐静脉内皮细胞（HUVEC）的粘附部分依赖于中性粒细胞上的CD11/CD18糖蛋白家族和HUVEC上的ICAM-1。使用亚单位特异性单克隆抗体（mab）在体外评估参与这种粘附的CD18异源二聚体。未刺激的中性粒细胞对il -1刺激的HUVEC的粘附被抗cd11a而非CD11b单克隆抗体显著抑制，而fmlp刺激的中性粒细胞对il -1刺激的HUVEC的粘附被抗cd11a和-CD11b均显著抑制。抗cd11a单克隆抗体（而非抗cd11b单克隆抗体）降低了纯化ICAM-1上未受刺激的中性粒细胞与对照蛋白糖蛋白上同样低水平的未受刺激中性粒细胞的粘附性。fMLP刺激显著增加中性粒细胞对纯化ICAM-1的附着，但对对照蛋白没有作用。抗cd11b单克隆抗体降低了这种对未受刺激的中性粒细胞的化学增强粘附性，并且与抗cd11a单克隆抗体联合使用降低了对对照蛋白的粘附性。本报告的结果表明，未受刺激的中性粒细胞表现出lfa -1依赖于ICAM-1的附着，趋化刺激通过mac -1依赖的过程增强了人中性粒细胞对ICAM-1的附着。
The adherence of human neutrophils to human umbilical vein endothelial cells (HUVEC) is partially dependent on the CD11/CD18 family of glycoproteins on the neutrophil and ICAM-1 on the HUVEC. The CD18 heterodimer involved in this adherence was evaluated in vitro using subunit-specific monoclonal antibodies (MAbs). The adherence of unstimulated neutrophils to IL-1-stimulated HUVEC was significantly inhibited by anti-CD11a but not CD11b MAbs, while the adherence of fMLP-stimulated neutrophils was significantly inhibited by both anti-CD11a and -CD11b. Anti-CD11a, but not anti-CD11b MAbs, reduced the adherence of unstimulated neutrophils on purified ICAM-1 to the same low level untreated neutrophils exhibited on a control protein, glycophorin. Stimulation with fMLP significantly increased neutrophil attachment to purified ICAM-1, but not the control protein. Anti-CD11b MAbs reduced this chemotactically augmented adherence to that of unstimulated neutrophils, and in combination with anti-CD11a MAbs reduced adherence to that on the control protein. The results in this report indicate that unstimulated neutrophils exhibit LFA-1-dependent attachment to ICAM-1, and chemotactic stimulation enhances the attachment of human neutrophils to ICAM-by A Mac-1-dependent process.