Influenza A and B viruses expressing altered NS1 proteins: A vaccine approach
Influenza A and B viruses expressing altered NS1 proteins: A vaccine approach
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DOI：
10.1073/pnas.070525997
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发表时间：
2000-04-11
影响因子：
11.1
通讯作者：
Palese, P
中科院分区：
文献类型：
--
作者：
Talon, J;Salvatore, M;Palese, P
我们提出了一种合理的方法来产生活病毒疫苗：改变病毒编码的I型IFN拮抗剂，以减弱毒力，同时保持免疫原性。我们利用流感病毒探索了这个概念。我们已经证实甲型流感病毒的NS1蛋白具有抗干扰素活性。我们现在提供的证据表明，甲型和乙型流感病毒编码改变的病毒NS1蛋白，在小鼠宿主中高度减毒，但对野生型病毒的攻击提供保护。
We propose a rational approach to the generation of live viral vaccines: alteration of virally encoded type I IFN antagonists to attenuate virulence while retaining immunogenicity. We have explored this concept by using the influenza virus. Previously we have shown that the NS1 protein of influenza A virus possesses anti-IFN activity. We now present evidence that influenza A and B viruses encoding altered viral NS1 proteins are highly attenuated in the mouse host, yet provide protection from challenge with wild-type viruses.