Progenitor targeting by adult stem cells in Ciona homeostasis, injury, and regeneration
Progenitor targeting by adult stem cells in Ciona homeostasis, injury, and regeneration
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DOI：
10.1016/j.ydbio.2018.09.005
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发表时间：
2019-04-15
影响因子：
2.7
通讯作者：
Jeffery, William R.
中科院分区：
文献类型：
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作者：
Jeffery, William R.
在海鞘玻璃海鞘中，口腔虹吸切断激活鳃囊中的成体干细胞壁龛，以分裂并将迁移祖细胞派遣到损伤部位的再生芽基。这项研究表明，源自鳃囊干细胞龛的祖细胞在体内平衡、伤口修复以及虹吸管和神经复合体（NC）的再生中发挥作用。在体内平衡过程中，祖细胞靶向咽部的气门，以取代纤毛细胞参与过滤器喂养。在单独或双虹吸管截肢后，祖细胞特异性地靶向口腔或心房虹吸管或两个虹吸管，并参与虹吸管环形肌纤维的替换。在口腔虹吸管损伤后，祖细胞靶向伤口部位，并且在某些情况下形成了额外虹吸管，尽管祖细胞靶向并不能预测额外虹吸管的诱导。在NC消融后，祖细胞特异性地靶向再生的NC，并提供新的脑和神经腺细胞的前体。鳃囊干细胞靶向的组织和器官在稳态和损伤过程中表现出凋亡。它的结论是，鳃囊祖细胞是多能的，并显示靶向特异性，与细胞凋亡在稳态生长，组织修复和再生。
In the ascidian Ciona intestinalis, oral siphon amputation activates adult stem cell niches in the branchial sac to divide and dispatch migratory progenitor cells to a regeneration blastema at the site of injury. This study shows that progenitor cells derived from branchial sac stem cell niches have roles in homeostasis, wound repair, and regeneration of the siphons and neural complex (NC). During homeostasis, progenitor cells targeted the pharyngeal stigmata to replace ciliated cells involved in filter feeding. After individual or double siphon amputations, progenitor cells specifically targeted the oral or atrial siphons or both siphons, and were involved in the replacement of siphon circular muscle fibers. After oral siphon wounding, progenitor cells targeted the wound sites, and in some cases a supernumerary siphon was formed, although progenitor cell targeting did not predict the induction of supernumerary siphons. Following NC ablation, progenitor cells specifically targeted the regenerating NC, and supplied the precursors of new brain and neural gland cells. The tissues and organs targeted by branchial sac stem cells exhibited apoptosis during homeostasis and injury. It is concluded that branchial sac progenitor cells are multipotent and show targeting specificity that is correlated with apoptosis during homeostatic growth, tissue repair, and regeneration.