Antifungal activity of essential oils and their synergy with fluconazole against drug-resistant strains of Aspergillus fumigatus and Trichophyton rubrum
Antifungal activity of essential oils and their synergy with fluconazole against drug-resistant strains of Aspergillus fumigatus and Trichophyton rubrum
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DOI：
10.1007/s00253-011-3152-3
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发表时间：
2011-05-01
影响因子：
5
通讯作者：
Ahmad, Iqbal
中科院分区：
文献类型：
--
作者：
Khan, Mohd Sajjad Ahmad;Ahmad, Iqbal
本研究的目的是筛选某些植物精油和活性化合物的抗真菌活性及其与氟康唑对耐药病原菌的体外相互作用。本工作采用的方法有：圆盘扩散法、肉汤大稀释法、时间杀伤法和棋盘格微量滴度法。采用气相色谱-质谱联用（GC-MS）法分析油的成分。采用透射电镜观察精油对真菌细胞结构的影响。试验真菌菌株对至少两种药物（氟康唑和伊曲康唑）表现出耐药性。在21种精油或活性化合物中，有10种具有良好的抗真菌活性。GC-MS分析显示，所使用的精油中存在主要活性化合物。肉桂醛对烟曲霉MTCC2550和红毛霉IOA-9表现出较好的抑菌活性和杀伤能力。肉桂醛与氟康唑的协同作用最强，可使氟康唑的最低抑菌浓度降低8倍。细胞壁和细胞膜的裂解区似乎是肉桂醛作用于真菌的地方。本研究强调了精油和活性化合物的广谱抗真菌活性及其与氟康唑对耐药真菌的协同作用。
The aim of this study was to screen certain plant essential oils and active compounds for antifungal activity and their in vitro interaction with fluconazole against drug-resistant pathogenic fungi. The methods employed in this work included disc diffusion, broth macrodilution, time kill methods and checkerboard microtiter tests. Oil compositions were evaluated by gas chromatography-mass spectrometry (GC-MS) analysis. Transmission electron microscopy was used to assess the effect of essential oils on cellular structures of test fungi. Test fungal strains exhibited resistance to at least two drugs (fluconazole and itraconazole). Among the 21 essential oils or active compounds tested, ten showed promising antifungal activity. GC-MS analysis revealed the presence of major active compounds in the essential oils used. Cinnamaldehyde showed the most promising antifungal activity and killing potency against Aspergillus fumigatus MTCC2550 and Trichophyton rubrum IOA-9. Cinnamaldehyde showed strongest synergy with fluconazole against A. fumigatus and T. rubrum by reducing the minimum inhibitory concentration of fluconazole up to 8-fold. Zones of lysis of the cell wall and cell membrane appeared to be where cinnamaldehyde acted on fungi. This study highlights the broad spectrum antifungal activity of essential oils and active compounds and their synergy with fluconazole against drug-resistant fungi.