Oral treatment with probiotic Lactobacillus johnsonii NCC533 (La1) for a specific part of the weaning period prevents the development of atopic dermatitis induced after maturation in model mice, NC/Nga
Oral treatment with probiotic Lactobacillus johnsonii NCC533 (La1) for a specific part of the weaning period prevents the development of atopic dermatitis induced after maturation in model mice, NC/Nga
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DOI：
10.1111/j.1365-2133.2006.07695.x
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发表时间：
2007-03-01
影响因子：
10.3
通讯作者：
Ushida, K.
中科院分区：
文献类型：
--
作者：
Inoue, R.;Nishio, A.;Ushida, K.
背景：益生菌对特应性皮炎的抑制作用已在人类婴儿中得到证实，但其机制仍不清楚。
目的：本研究旨在展示在小鼠断奶期施用益生菌对肠道分泌型免疫球蛋白A（sIgA）产生以及对后期特应性皮炎（AD）发展的影响。
方法：使用小鼠模型（Balb/c）评估在断奶期间施用约氏乳杆菌NCC533（La1）的效果。根据粪便IgA的变化将小鼠断奶期分为三个阶段。在每个阶段施用La1并评估粪便IgA的变化。在接下来的实验中，通过使用人类AD的NC/Nga小鼠模型进一步评估在第2阶段施用La1对成熟后宿主免疫的影响。
结果：在每个阶段施用La1对sIgA的产生都有明显影响。但只有在第2阶段施用La1时，sIgA的产生才受到正向刺激。在第2阶段初次施用La1可显著预防从6周龄开始由螨抗原诱导的AD发展。与对照小鼠相比，类似AD的病变明显较轻，组织学观察显示，施用La1的小鼠表皮和上层真皮外观几乎正常。
结论：本研究表明，在断奶期的特定阶段初次施用La1通过调节或加速肠道免疫反应，可有效预防或抑制成熟后AD的发展。
Background The inhibitory effect of probiotic bacteria on atopic dermatitis has been shown in human infants, but the mechanism is still unclear.Objective This study aimed to show the effects of the administration of a probiotic during the weaning period in mouse models on production of the intestinal secretory IgA (sIgA) and on the development of atopic dermatitis (AD) in later life.Methods The effects of the administration of Lactobacillus johnsonii NCC533 (La1) during weaning were evaluated using a mouse model (Balb/c). The weaning period of mice was divided into three phases according to the evolution of faecal IgA. La1 was administered in each phase and the evolution of the faecal IgA was estimated. In the next experiment, the effect of the administration of La1 in phase 2 on host immunity after maturation was further assessed by using the model NC/Nga mouse for human AD.Results Administration of La1 in each phase showed a distinct effect on the production of sIgA. But sIgA production was only positively stimulated when La1 was administrated in phase 2. The development of AD induced by mite antigen from 6 weeks old was significantly prevented by the primary administration of La1 in phase 2. AD-like lesions were significantly milder than those of the control mice, and histological observations showed an almost normal appearance of the epidermis and upper dermis of the mice treated with La1.Conclusion This study suggested that the primary administration of La1 in a specific part of the weaning period is effective in preventing or inhibiting the development of AD after maturation by modulating or accelerating the gut immune response.