Hemagglutinin Gene Shuffling among Clostridium botulinumSerotypes C and D Yields Distinct Sugar Recognition of the Botulinum Toxin Complex
Hemagglutinin Gene Shuffling among Clostridium botulinumSerotypes C and D Yields Distinct Sugar Recognition of the Botulinum Toxin Complex
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肉毒杆菌血清型 C 和 D 之间的血凝素基因改组产生肉毒杆菌毒素复合物的独特糖识别
DOI：
10.1093/femspd/ftv054
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Sagane Y
中科院分区：
文献类型：
--
作者：
Miyata K;Suzuki T;Hayashi S;Miyashita S;Ohyama T;Niwa K;Watanabe T;Sagane Y
肉毒梭菌菌株产生由肉毒神经毒素（BoNT）、无毒非血凝素和三种不同血凝素（HA-70、HA-33和HA-17）组成的大尺寸毒素复合物（TC）。HA组分以糖链依赖性方式增强穿过肠细胞壁的毒素递送。在这里，我们表征了血清型D菌株1873（D-1873）肉毒杆菌L-TC的糖识别。大多数由血清型C和D菌株产生的L-TC通过HA-33与细胞表面唾液酸寡糖之间的相互作用与细胞结合。然而，与先前报道的由血清型C菌株Yoichi（C-Yoichi）产生的L-TC一样，D-1873 L-TC仅与经神经氨酸酶处理的细胞结合，表明它们识别脱唾液酸寡糖。D-1873 HA-33氨基酸序列与C-Yoichi相似，但与大多数血清型C和D HA-33的相似性较低。12个血清型C和D菌株的TC组分一级结构的比较表明，至少存在三种类型的HA-33基因，并且这些基因在血清型C和D菌株之间独立于BoNT血清型而被改组。这种改组产生血清型C和D肉毒杆菌TC的不同糖识别。
Clostridium botulinumstrains produce a large-sized toxin complex (TC) that is composed of botulinum neurotoxin (BoNT), non-toxic non-hemagglutinin and three different hemagglutinins (HA-70, HA-33 and HA-17). HA components enhance toxin delivery across the intestinal cell wall in a sugar chain-dependent manner. Here we characterized the sugar recognition of serotype D strain 1873 (D-1873) botulinum L-TC. Most L-TCs produced by serotype C and D strains bind to cells via interactions between HA-33 and cell surface sialo-oligosaccharides. However, like the previously reported L-TC produced by serotype C strain Yoichi (C-Yoichi), D-1873 L-TC binds only to cells that have been treated with neuraminidase, indicating that they recognize asialo-oligosaccharides. The D-1873 HA-33 amino acid sequence is similar to that of C-Yoichi, but had lower similarity to the majority of serotype C and D HA-33s. A comparison of TC component primary structures for 12 serotype C and D strains suggested that at least three types of HA-33 genes exist, and these are shuffled among the serotype C and D strains independently of BoNT serotype. This shuffling produces the distinct sugar recognition of serotype C and D botulinum TCs.