The Bio-Synthesis of Three Metal Oxide Nanoparticles (ZnO, MnO(2), and MgO) and Their Antibacterial Activity Against the Bacterial Leaf Blight Pathogen.
The Bio-Synthesis of Three Metal Oxide Nanoparticles (ZnO, MnO(2), and MgO) and Their Antibacterial Activity Against the Bacterial Leaf Blight Pathogen.
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三种金属氧化物纳米颗粒（ZnO，MNO（2）和MGO）的生物合成及其针对细菌叶片叶片病原体的抗菌活性。
DOI：
10.3389/fmicb.2020.588326
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发表时间：
2020
影响因子：
5.2
通讯作者：
Li B
中科院分区：
文献类型：
--
作者：
Ogunyemi SO;Zhang M;Abdallah Y;Ahmed T;Qiu W;Ali MA;Yan C;Yang Y;Chen J;Li B
水稻白白叶枯病菌（Xoo）是引起水稻白叶枯病（BLB）的致病性最强的病原菌。然而，Xoo的化学或抗生素抗性的积累需要开发其替代控制。在这项研究中，我们生物合成三种金属氧化物纳米粒子（ZnO，MnO 2，MgO）使用根生细菌多粘类芽孢杆菌菌株Sx 3作为还原剂。通过紫外可见光谱、XRD、FTIR、EDS、SEM和TEM分析证实并表征了纳米颗粒的生物合成。纳米颗粒的UV维斯反射率在385、230和230 nm处具有峰值，ZnO、MnO 2和MgO的平均微晶颗粒尺寸分别为62.8、18.8和10.9 nm。纳米ZnO、MnO 2和MgO对Xoo菌株GZ 0006的抑菌效果均达到显著水平，浓度为16.0 μg/ml时，抑菌面积分别为17、13和13 mm，生物膜形成量分别减少了74.5、74.4和80.2%。此外，根据纳米颗粒的抗菌效率和细胞壁的畸形现象，推断纳米颗粒的作用机制与活性氧有关。总的来说，一个有吸引力的和生态友好的生物ZnO，MnO 2和MgO纳米粒子成功地生产。总之，结果表明，纳米颗粒对水稻BLB病具有优异的抗菌效果，同时增加了生长参数和水稻生物量。总之，合成的纳米颗粒可以作为一种替代的安全措施，在对抗Xoo的抗药性。
Xanthomonas oryzae pv. oryzae (Xoo) is the most infectious pathogen of rice, which causes bacterial leaf blight (BLB) disease. However, the accumulation of chemical or antibiotic resistance of Xoo necessitate the development of its alternative control. In this study, we biologically synthesize three metal oxide nanoparticles (ZnO, MnO2, and MgO) using rhizophytic bacteria Paenibacillus polymyxa strain Sx3 as reducing agent. The biosynthesis of nanoparticles was confirmed and characterized by using UV-vis spectroscopy, XRD, FTIR, EDS, SEM, and TEM analysis. The UV Vis reflectance of the nanoparticle had peaks at 385, 230, and 230 nm with an average crystallite particle size 62.8, 18.8, and 10.9 nm for ZnO, MnO2, and MgO, respectively. Biogenic ZnO, MnO2, and MgO nanoparticles showed substantial significant inhibition effects against Xoo strain GZ 0006 at a concentration of 16.0 μg/ml, for which the antagonized area was 17, 13, and 13 mm and the biofilm formation was decreased by 74.5, 74.4, and 80.2%, respectively. Moreover, the underlining mechanism of nanoparticles was inferred to be in relation to the reactive oxygen species based on their antibacterial efficiency and the deformity in the cell wall phenomenon. Overall, an attractive and eco-friendly biogenic ZnO, MnO2, and MgO nanoparticles were successfully produced. Altogether, the results suggest that the nanoparticles had an excellent antibacterial efficacy against BLB disease in rice plants, together with the increase in growth parameter and rice biomass. In conclusion, the synthesized nanoparticles could serve as an alternative safe measure in combatting the antibiotic-resistant of Xoo.
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影响因子：
3
作者：
Borase, Hemant P.;Salunke, Bipinchandra K.;Patil, Satish V.
通讯作者：
Patil, Satish V.
影响因子：
2.4
作者：
Chen, JC;Lin, ZH;Ma, XX
通讯作者：
Ma, XX
影响因子：
5.8
作者：
Jha, Anal K.;Prasad, K.
通讯作者：
Prasad, K.
影响因子：
2.5
作者：
Jin, Tony;He, Yiping
通讯作者：
He, Yiping
影响因子：
5.2
作者：
Cai L;Chen J;Liu Z;Wang H;Yang H;Ding W
通讯作者：
Ding W