Collagen synthesis in virus-transformed cells.
Collagen synthesis in virus-transformed cells.
复制标题
病毒转化细胞中的胶原蛋白合成。
DOI：
10.1111/j.1749-6632.1985.tb51168.x
复制
发表时间：
1985
影响因子：
5.2
通讯作者：
Boettiger,D
中科院分区：
文献类型：
--
作者：
Adams,SL;Pacifici,M;Focht,RJ;Allebach,ES;Boettiger,D
病毒转化对培养细胞生物合成程序最显着的影响之一是胶原蛋白合成减少，这通常与其他细胞外基质蛋白合成减少一起发生。由此产生的正常细胞外基质的损失与转化细胞中观察到的细胞形状和行为的巨大变化有关。一些实验室已经开始研究胶原蛋白合成减少的分子基础。在本文中，我们总结了这些结果，并描述了我们实验室的最新实验，这些实验详细检查了转化对皮肤成纤维细胞和椎骨软骨细胞（鸡胚间充质细胞的两种终末分化群体）中胶原蛋白合成的影响。这些细胞合成非常不同类型的细胞外基质，并在胶原蛋白基因表达方面对病毒转化表现出不同的反应。这些实验的结果使我们提出，I1 型胶原蛋白的合成（几乎只发生在终末分化的软骨细胞中）主要在转录水平上受到控制。相比之下，I 型胶原蛋白的合成发生在许多细胞中（但不在软骨细胞中），在转录后和转录水平上受到控制。此外，I 型和 I1 型胶原蛋白基因的表达均受到细胞附着介导的细胞形状改变的调节。这些观察结果对于正常发育过程中胶原蛋白基因表达的控制以及病毒转化对胶原蛋白基因表达的影响具有重要意义。此外，它们可能会增加我们对细胞形状和行为以及胶原蛋白和细胞外基质其他成分的合成之间复杂相互作用的理解。 a 这项工作得到了美国国立卫生研究院的 GM-28840、GM-09590、CA-16502、HD-07152 和 AG-02758 号拨款的支持。
One of the most striking effects of viral transformation on the biosynthetic program of cultured cells is the reduced synthesis of collagen, which often occurs together with decreased synthesis of other extracellular matrix proteins. The resulting loss of the normal extracellular matrix is associated with the dramatic changes in cell shape and behavior observed in transformed cells. Several laboratories have begun to examine the molecular basis for the reduction of collagen synthesis. In this paper we summarize those results, and describe recent experiments from our laboratories which examine in detail the effects of transformation on collagen synthesis in skin fibroblasts and vertebral chondrocytes, two terminally differentiated populations of mesenchymal cells from chick embryos. These cells synthesize very different types of extracellular matrix and display different responses to viral transformation in terms of collagen gene expression. The results of these experiments have led us to suggest that synthesis of type I1 collagen, which occurs almost exclusively in terminally differentiated chondrocytes, is controlled primarily at the level of transcription. In contrast, synthesis of type I collagen, which occurs in many cells (but not in chondrocytes), is controlled at post-transcriptional as well as transcriptional levels. Furthermore, expression of both the types I and I1 collagen genes is modulated by alterations in cell shape which are mediated by cell attachment. These observations have important implications for the control of collagen gene expression during normal development, as well as for the effects of viral transformation on collagen gene expression. In addition, they may increase our understanding of the complex interplay between cell shape and behavior and synthesis of collagen and other components of the extracellular matrix. aThis work was supported by grant nos GM-28840, GM-09590, CA-16502, HD-07152, and AG-02758 from the National Institutes of Health.