Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing
Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing
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DOI：
10.1016/j.vetpar.2004.04.008
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发表时间：
2004-07-14
影响因子：
2.6
通讯作者：
Orecchia, P
中科院分区：
文献类型：
--
作者：
Berrilli, F;Di Cave, D;Orecchia, P
为了调查来自意大利家畜和农场动物的十二指肠贾第虫的基因型，通过SSU-rRNA基因测序对21株贾第虫分离株（17株来自狗，1株来自猫，3株来自奶牛）进行了遗传特征分析。在犬中，76.5%的分离株表现出犬特有的基因型（集合C、D和C/D混合集合），23.5%的分离株表现出潜在的人畜共患病基因型（集合A和A/C混合集合）。猫分离物属于A组合，而小牛分离物的序列被发现对应于有蹄类家畜基因型，即组合E。这些发现表明，人畜共患病基因型感染人类可能是低流行病学意义，虽然可能的。本研究首次对G.来自意大利的家畜和农场动物中的马立克氏菌基因型。(C)2004 Elsevier B. V.保留所有权利。
In order to investigate the genotypes of Giardia duodenalis from domestic and farm animals in Italy, 21 Giardia isolates, 17 from dogs, 1 from cat and 3 from dairy calves, were genetically characterised by SSU-rRNA gene sequencing. Among dogs, 76.5% of isolates showed the dog-specific genotypes (Assemblages C, D and C/D mixed Assemblage) and 23.5% exhibit potential zoonotic genotypes (Assemblage A and A/C mixed Assemblages). The cat isolate belonged to assemblage A, whereas the sequences among the isolates from calves were found to correspond to hoofed-livestock genotype, namely Assemblage E. These findings suggest that infection of humans by zoonotic genotypes from domestic animals could be of low epidemiological significance, although possible. The present study represents the first contribute to the knowledge of G. duodenalis genotypes in domestic and farm animals from Italy. (C) 2004 Elsevier B.V. All rights reserved.