Anti-F(ab')2 antibodies in thrombocytopenic patients at risk for acquired immunodeficiency syndrome.
Anti-F(ab')2 antibodies in thrombocytopenic patients at risk for acquired immunodeficiency syndrome.
复制标题
DOI：
10.1172/jci112498
复制
发表时间：
1986-06
期刊：
影响因子：
--
通讯作者：
J. Yu;E. Lennette;S. Karpatkin
中科院分区：
文献类型：
--
作者：
J. Yu;E. Lennette;S. Karpatkin
对22例有获得性免疫缺陷综合征(AIDS)危险因素或合并AIDS的同性恋者或吸毒者进行了抗免疫球蛋白抗体和循环免疫复合物的检测(其中20例为血小板减少症，6例为AIDS)。循环免疫复合体水平是正常受试者的10倍。在同性恋者和吸毒者中均可检测到抗F(ab‘)2抗体。在16例同性恋患者中，7例具有中等至显著滴度的抗F(ab‘)2抗体，对自体、同源和对照F(ab’)2片段具有广泛的反应性。另外三个对一个或两个F(ab‘)2片段显示出有限的反应活性。其余6名患者均为阴性。6例吸毒者中有6例有抗F(ab‘)2抗体，其中5例反应有限，1例广泛反应。相比之下，在6名自身免疫性血小板减少患者中，既没有检测到循环免疫复合体升高，也没有检测到抗F(ab‘)2抗体。抗F(ab‘)2抗体可从血清或循环免疫复合物中亲和纯化。抗F(ab‘)2反应性与循环免疫复合体水平相关，r=0.83，P<0.01。
22 homosexual or narcotic addict patients at risk for acquired immunodeficiency syndrome (AIDS) or with AIDS, were studied for the presence of antiimmunoglobulin antibodies and circulating immune complexes (20 were thrombocytopenic, 6 had AIDS). Circulating immune complex levels were 10-fold higher than levels in normal subjects. IgG anti-F(ab')2 antibodies were noted in homosexual as well as narcotic addict patients. Of 16 homosexual patients, 7 had IgG anti-F(ab')2 antibody of moderate to marked titer with broad reactivity against autologous, homologous, and control F(ab')2 fragments. Three others demonstrated limited reactivity against one or two F(ab')2 fragments. The remaining six patients were negative. Six of six narcotic addict patients had IgG anti-F(ab')2 antibody, five with limited reactivity, one with broad reactivity. In contrast, neither elevated circulating immune complexes nor anti-F(ab')2 antibodies were detectable in six autoimmune thrombocytopenic patients. Anti-F(ab')2 antibody could be affinity purified from serum or circulating immune complexes. Anti-F(ab')2 reactivity correlated with circulating immune complex levels, r = 0.83, P less than 0.01.