A receptor-like protein acts as a specificity switch for the regulation of stomatal development.
A receptor-like protein acts as a specificity switch for the regulation of stomatal development.
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DOI：
10.1101/gad.297580.117
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发表时间：
2017-05-01
影响因子：
10.5
通讯作者：
Chai J
中科院分区：
文献类型：
--
作者：
Lin G;Zhang L;Han Z;Yang X;Liu W;Li E;Chang J;Qi Y;Shpak ED;Chai J
在拟南芥中，气孔模式由ERECTA家族（ERf）受体激酶（RKs）、受体样蛋白（RLP）太多口（TMM）和表皮模式因子（EPF）肽指定。在这里，Lin等人表明，TMM决定了ERfs对不同EPFs的感知的特异性，因此作为调节ERfs活性的特异性开关。气孔是允许植物和环境之间进行气体交换的微观开口。在拟南芥中，气孔模式由ERECTA家族（ERf）受体激酶（RKs）、受体样蛋白（RLP）太多口（TMM）和表皮模式因子（EPF）肽指定。在这里，我们表明，TMM和ER或ERL 1（ERL 1）形成组成型复合物，识别EPF 1和EPF 2，但单一的ERfs不。TMM与ERL 1的相互作用产生了识别EPF 1和EPF 2的结合口袋，表明组成型TMM-ERf复合物作为EPF 1和EPF 2的受体发挥作用。EPFL 9与EPF 1和EPF 2竞争结合ERf-TMM复合物。然而，EPFL 4和EPFL 6被单个ERf识别，而不需要TMM。与EPF 1，2相反，EPFL 4，6与ERF的相互作用在TMM的存在下大大降低。两者合计，我们的数据表明，TMM规定的ERfs的特异性不同的EPFs的感知，从而作为一个特异性开关的ERfs的活动的调节功能。
In Arabidopsis, stomatal patterning is specified by the ERECTA family (ERf) receptor kinases (RKs), the receptor-like protein (RLP) TOO MANY MOUTHS (TMM), and EPIDERMAL PATTERNING FACTOR (EPF) peptides. Here, Lin et al. show that TMM dictates the specificity of ERfs for perception of different EPFs, thus functioning as a specificity switch for the regulation of the activities of ERfs. Stomata are microscopic openings that allow for the exchange of gases between plants and the environment. In Arabidopsis, stomatal patterning is specified by the ERECTA family (ERf) receptor kinases (RKs), the receptor-like protein (RLP) TOO MANY MOUTHS (TMM), and EPIDERMAL PATTERNING FACTOR (EPF) peptides. Here we show that TMM and ER or ER-LIKE1 (ERL1) form constitutive complexes, which recognize EPF1 and EPF2, but the single ERfs do not. TMM interaction with ERL1 creates a binding pocket for recognition of EPF1 and EPF2, indicating that the constitutive TMM–ERf complexes function as the receptors of EPF1 and EPF2. EPFL9 competes with EPF1 and EPF2 for binding to the ERf–TMM complex. EPFL4 and EPFL6, however, are recognized by the single ERfs without the requirement of TMM. In contrast to EPF1,2, the interaction of EPFL4,6 with an ERf is greatly reduced in the presence of TMM. Taken together, our data demonstrate that TMM dictates the specificity of ERfs for the perception of different EPFs, thus functioning as a specificity switch for the regulation of the activities of ERfs.