Deep Sequencing of the Fruit Transcriptome and Lipid Accumulation in a Non-Seed Tissue of Chinese Tallow, a Potential Biofuel Crop
Deep Sequencing of the Fruit Transcriptome and Lipid Accumulation in a Non-Seed Tissue of Chinese Tallow, a Potential Biofuel Crop
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DOI：
10.1093/pcp/pcv181
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发表时间：
2016-01-01
影响因子：
4.9
通讯作者：
Singh, Surinder P.
中科院分区：
文献类型：
--
作者：
Divi, Uday K.;Zhou, Xue-Rong;Singh, Surinder P.
中国牛油（Triadica sebifera）是一种有价值的油籽树，可以在各种条件下生长而不竞争粮食生产，是一种有前途的生物燃料原料。果实的独特之处在于它们分别含有存在于油脂和种子层中的饱和脂肪和不饱和脂肪。油脂层的研究很少，只被认为是种子分泌的外部脂肪沉积。在这项研究中，我们表明，脂肪实际上是一种非种子细胞组织，能够合成甘油三酯。除种子外，脂质合成和储存机制的知识是有限的，但对于生产富含油脂的生物质作物是必不可少的。在这里，我们描述了中国牛脂果皮、牛脂和种子组织中产生的带注释的转录组组装。最后的组装被功能注释，允许候选基因的鉴定和脂质途径的重建。研究人员发现，与种子组织中表达的转录因子基因wrled1 （WRI1）和脂滴相关蛋白基因不同，它们在脂肪组织中具有活性，支撑了脂肪组织中油脂合成和包装的模式。我们的数据已经建立了一个优秀的知识库，可以为工程非种子组织积累大量油提供遗传和生化方面的见解。除了注释转录本的大数据集外，该研究还提供了基于基因的简单序列重复和单核苷酸多态性标记。
Chinese tallow (Triadica sebifera) is a valuable oilseed-producing tree that can grow in a variety of conditions without competing for food production, and is a promising biofuel feedstock candidate. The fruits are unique in that they contain both saturated and unsaturated fat present in the tallow and seed layer, respectively. The tallow layer is poorly studied and is considered only as an external fatty deposition secreted from the seed. In this study we show that tallow is in fact a non-seed cellular tissue capable of triglyceride synthesis. Knowledge of lipid synthesis and storage mechanisms in tissues other than seed is limited but essential to generate oil-rich biomass crops. Here, we describe the annotated transcriptome assembly generated from the fruit coat, tallow and seed tissues of Chinese tallow. The final assembly was functionally annotated, allowing for the identification of candidate genes and reconstruction of lipid pathways. A tallow tissue-specific paralog for the transcription factor gene WRINKLED1 (WRI1) and lipid droplet-associated protein genes, distinct from those expressed in seed tissue, were found to be active in tallow, underpinning the mode of oil synthesis and packaging in this tissue. Our data have established an excellent knowledge base that can provide genetic and biochemical insights for engineering non-seed tissues to accumulate large amounts of oil. In addition to the large data set of annotated transcripts, the study also provides gene-based simple sequence repeat and single nucleotide polymorphism markers.