Alleviation effect of conjugated linoleic acid on estradiol benzoate induced fatty liver hemorrhage syndrome in Hy-line male chickens
Alleviation effect of conjugated linoleic acid on estradiol benzoate induced fatty liver hemorrhage syndrome in Hy-line male chickens
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共轭亚油酸对苯甲酸雌二醇所致海兰公鸡脂肪肝出血综合征的缓解作用
DOI：
10.1093/jas/skad045
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发表时间：
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影响因子：
3.3
通讯作者：
Xuelan Liu
中科院分区：
文献类型：
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作者：
Anqi Wang;Kexin Zhang;Chunyan Fu;Changming Zhou;Zhengui Yan;Xuelan Liu
本研究旨在探讨共轭亚油酸(CLA)能否减轻苯甲酸雌二醇肌内注射诱发的蛋鸡脂肪肝出血综合征(FLHS)。选用100只雄性海兰白鸡，随机分为对照组(CON)和苯甲酸雌二醇组(E)，两组饲喂相同的基础日粮。每两天注射苯甲酸雌二醇2 mg/kg，共7次后，E组鸡出现肝脏肿大、出血、脂肪变性等症状。然后，E组的一半鸡接受8周的添加含有5000 mg/kg CLA的日粮。形态学观察、苏木精-伊红染色和油红O染色结果表明，共轭亚油酸可减轻FLHS雏鸡肝脏肿大、出血和脂质堆积。此外，我们还检测了肝功能和脂代谢指标，包括ALT、AST、TG、TCH、HDL-C和LDL-C，进一步表明CLA减轻了FLHS鸡的血清和肝脏代谢紊乱。CLA通过调节CD36、Acc、Fas、SCD1、DGAT2、LIPE、ATGL、CPT1a、SREBP-1c、SREBP-2、PPARγ和PPARα的基因表达，从机制上抑制肝脂生成、胆固醇合成和甘油三酯的积累，增加甘油三酯的水解率。此外，共轭亚油酸还能减轻肝脏氧化应激反应，抑制NF-κB信号通路介导的炎症反应。综上所述，共轭亚油酸可调节脂类代谢，从而进一步减轻氧化应激和炎症反应，从而减轻雌激素诱导的鸡FLHS。
The purpose of this study was to explore whether conjugated linoleic acid (CLA) could alleviate fatty liver hemorrhagic syndrome (FLHS) induced by estradiol benzoate intramuscular injection in laying hens. One hundred male Hy-Line white chickens were randomly divided into two groups, namely, the control (CON) and estradiol benzoate (E) groups, and both groups were fed the same basal diet. After injections of estradiol benzoate at 2 mg/kg every two days for a total of 7 times, chickens in the E group showed FLHS symptoms, including liver enlargement, hemorrhage, and steatosis. Then half of the chickens in the E group received an additional diet containing 5000 mg/kg CLA for 8 weeks. The results of morphological observations, hematoxylin and eosin staining, and Oil Red O staining showed that CLA alleviated liver enlargement, hemorrhage, and lipid accumulation in FLHS chickens. In addition, we measured liver function and lipid metabolism indicators, including ALT, AST, TG, TCH, HDL-C, and LDL-C, which further suggested that CLA mitigated the disturbance of serum and liver metabolism in FLHS chickens. Mechanistically, CLA inhibited hepatic de novo lipogenesis, cholesterol synthesis, and TG accumulation and increased TG hydrolysis in FLHS chickens by regulating the gene expression of CD36, ACC, FAS, SCD 1, DGAT2, LIPE, ATGL, CPT1A, SREBP-1c, SREBP-2, PPARγ, and PPARα. Furthermore, CLA ameliorated hepatic oxidative stress and inhibited NF-κB signaling pathway-mediated inflammation in FLHS chickens. In conclusion, CLA regulated lipid metabolism, thus further alleviating oxidative stress and inflammation to alleviate FLHS induced by estrogen in chickens.