An adenylyl cyclase, CyaB, acts as an osmosensor in Myxococcus xanthus
An adenylyl cyclase, CyaB, acts as an osmosensor in Myxococcus xanthus
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DOI：
10.1128/jb.187.10.3593-3598.2005
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发表时间：
2005-05-01
影响因子：
3.2
通讯作者：
Takegawa, K
中科院分区：
文献类型：
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作者：
Kimura, Y;Ohtani, M;Takegawa, K
我们以前已经报道过黄色粘球菌的受体型腺苷酸环化酶(CyaA)主要在孢子萌发期间经历渗透传感器(Y.Kimura等人，J.细菌素)。184：3578-3585,2002)。在本研究中，我们克隆了另一个受体型腺苷酸环化酶基因(CyaB)，并对其编码蛋白的功能进行了鉴定。CyaB的破坏产生了一个突变体，在高离子(NaCI)或高非离子(蔗糖)渗透压下表现出生长迟缓。当0.15M NaCI刺激营养细胞时，CyaB突变细胞内cAMP水平的增加低于野生型细胞。在非离子渗透胁迫下，cyaB突变体的孢子萌发率降低，但其萌发率显著高于eyaA突变体。
We have previously reported that a receptor-type adenylyl cyclase (CyaA) of Myxococcus xanthus undergoes an osmosensor mainly during spore germination (Y. Kimura et al., J. Bacteriol. 184:3578-3585, 2002). In the present study, we cloned another receptor-type adenylyl cyclase gene (cyaB) and characterized the function of the cyaB-encoded protein. Disruption of cyaB generates a mutant that showed growth retardation at high ionic (NaCI) or high nonionic (sucrose) osmolarity. When vegetative cells were stimulated with 0.15 M NaCI, the increases in intracellular cyclic AMP levels of cyaB mutant cells were lower than those of wild-type cells. Under nonionic osmostress, the cyaB mutant exhibited reduced spore germination; however, the germination rate of the cyaB mutant was significantly higher than that of the eyaA mutant.