The bacterial community in potato is recruited from soil and partly inherited across generations
The bacterial community in potato is recruited from soil and partly inherited across generations
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DOI：
10.1371/journal.pone.0223691
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发表时间：
2019-11-08
期刊：
影响因子：
3.7
通讯作者：
Mitter, Birgit
中科院分区：
文献类型：
--
作者：
Buchholz, Franziska;Antonielli, Livio;Mitter, Birgit
人们已经做出了很大的努力来了解马铃薯植物和根际的细菌群落。研究主要集中在环境和植物基因型对细菌群落结构和动态的影响，而对细菌群落的起源和组装知之甚少，特别是在马铃薯块茎中。然而，块茎微生物群可能特别令人感兴趣，因为它可以在作物质量中发挥重要作用，例如储存稳定性。在这里，我们使用16S rRNA基因扩增子测序研究细菌群落，殖民块茎的奥地利马铃薯生产中常用的三代以上，生长在不同的土壤中的不同的马铃薯品种。测序数据的统计分析表明，马铃薯块茎的细菌群落随着世代的变化而变化，并且变得与土壤细菌群落更相似，而马铃薯品种对细菌组合的影响随着时间的推移而失去意义。不同块茎部位的细菌群落差异不显著，但土壤细菌群落对块茎微生物区系组成的影响差异显著。另外，在随后的块茎生成中OTU的存在表明块茎微生物群的子集的垂直传播。所有块茎世代和所有马铃薯品种都共有四个OTU。总之，我们得出结论，马铃薯块茎的微生物群是从土壤中招募的，在很大程度上独立于植物品种。此外，马铃薯块茎中的细菌聚集体由从一个块茎世代传递到下一个块茎世代的细菌和从土壤中招募的细菌组成。
Strong efforts have been made to understand the bacterial communities in potato plants and the rhizosphere. Research has focused on the effect of the environment and plant genotype on bacterial community structures and dynamics, while little is known about the origin and assembly of the bacterial community, especially in potato tubers. The tuber microbiota, however, may be of special interest as it could play an important role in crop quality, such as storage stability. Here, we used 16S rRNA gene amplicon sequencing to study the bacterial communities that colonize tubers of different potato cultivars commonly used in Austrian potato production over three generations and grown in different soils. Statistical analysis of sequencing data showed that the bacterial community of potato tubers has changed over generations and has become more similar to the soil bacterial community, while the impact of the potato cultivar on the bacterial assemblage has lost significance over time. The communities in different tuber parts did not differ significantly, while the soil bacterial community showed significant differences to the tuber microbiota composition. Additionally, the presence of OTUs in subsequent tuber generation points to vertical transmission of a subset of the tuber microbiota. Four OTUs were common to all tuber generations and all potato varieties. In summary, we conclude that the microbiota of potato tubers is recruited from the soil largely independent from the plant variety. Furthermore, the bacterial assemblage in potato tubers consists of bacteria transmitted from one tuber generation to the next and bacteria recruited from the soil.