Mediators of estradiol-stimulated mitosis in the rat uterine luminal epithelium.
Mediators of estradiol-stimulated mitosis in the rat uterine luminal epithelium.
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DOI：
10.1210/endo.139.3.5794
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发表时间：
1998-03
期刊：
影响因子：
4.8
通讯作者：
Zhiming Zhang;Jane Laping;S. R. Glasser;Peter W. Day;J. Mulholland;J. Mulholland
中科院分区：
文献类型：
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作者：
Zhiming Zhang;Jane Laping;S. R. Glasser;Peter W. Day;J. Mulholland;J. Mulholland
雌二醇治疗，刺激细胞分裂在大鼠子宫上皮细胞，肝素结合表皮生长因子（HB-EGF），细胞周期蛋白D1，细胞周期蛋白B1信使RNA（mRNA）在这些细胞中的体内表达的影响已被检查使用核糖核酸酶保护试验。治疗后2小时和24小时，Eglycoprotein引起HB-EGF稳态水平的显著增加。细胞周期蛋白D1 mRNA水平升高后8和10小时，雌二醇治疗，相应的有丝分裂周期的G1期，和细胞周期蛋白B1 mRNA表达后16-24小时，雌二醇治疗，相应的大鼠子宫上皮细胞周期的G2和M期。雌二醇刺激的HB-EGF mRNA的增加不受放线菌酮治疗的影响，但受到雌激素拮抗剂化合物ICI 164，384的抑制，表明雌激素刺激的HB-EGF mRNA的增加是大鼠子宫上皮对雌二醇的主要雌激素受体介导的反应。孕激素治疗，即阻止上皮细胞在G1的周期，抑制HB-EGF mRNA水平低于卵巢切除大鼠中观察到的。这些结果表明，HB-EGF介导的调节作用，雌二醇和孕酮对大鼠子宫上皮细胞增殖的G1期分子，如细胞周期蛋白D1的生产的影响。
The effects of estradiol treatment, which stimulates cell division in rat uterine epithelial cells, on the in vivo expression of heparin-binding epidermal growth factor (HB-EGF), cyclin D1, and cyclin B1 messenger RNA (mRNA) in these cells have been examined using ribonuclease protection assays. Estradiol gave rise to significant increases in steady state levels of HB-EGF 2 and 24 h after treatment. Cyclin D1 mRNA levels were elevated 8 and 10 h after estradiol administration, corresponding to the G1 phase of the mitotic cycle, and cyclin B1 mRNA was only expressed 16-24 h after estradiol treatment, which corresponds to the G2 and M phases of the rat uterine epithelial cell cycle. Estradiol-stimulated increases in HB-EGF mRNA were not affected by treatment with cycloheximide, but were inhibited by the estrogen antagonist compound, ICI 164,384, demonstrating that the estrogen-stimulated increase in HB-EGF mRNA is a primary, estrogen receptor-mediated response of rat uterine epithelium to estradiol. Progesterone treatment, which blocks epithelial cells in G1 of the cycle, suppressed levels of HB-EGF mRNA below those observed in ovariectomized rats. These results indicate that HB-EGF mediates the regulatory effects of both estradiol and progesterone on rat uterine epithelial cell proliferation through an effect on the production of G1 phase molecules such as cyclin D1.